Health Effects and Life Stage Sensitivities in Zebrafish Exposed to an Estrogenic Wastewater Treatment Works Effluent.
Health Effects and Life Stage Sensitivities in Zebrafish Exposed to an Estrogenic Wastewater Treatment Works Effluent.
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DOI：
10.3389/fendo.2021.666656
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发表时间：
2021
影响因子：
5.2
通讯作者：
Tyler CR
中科院分区：
文献类型：
--
作者：
Cooper R;David A;Lange A;Tyler CR
据报道，鱼类暴露于污水处理工程（WwTW）流出物会产生广泛的健康影响，包括雄性的雌性化反应。然而，这些接触研究大多评估了急性健康影响，而慢性接触影响则不太确定。利用雌激素响应元件-绿色荧光蛋白(re - gfp)-Casper转基因斑马鱼，我们研究了暴露于雌激素性废水和合成雌激素17α-炔雌醇（EE2）的慢性健康影响和生命阶段敏感性。暴露于污水处理废水（100%）和10 ng/L（标称）EE2会延迟雄性鱼的睾丸成熟，但会加速雌性鱼的卵巢发育。暴露于50%和100%的废水以及10 ng/L的EE2也会导致性别比例偏向女性。长期暴露于100%废水中的ERE-GFP-斑马鱼在靶组织和发育生命阶段表现出不同的绿色荧光蛋白（GFP）表达模式，反映了废水中的雌激素含量。在整个研究过程中，gfp和卵黄蛋白原（vtg） mRNA的诱导与流出物中甾体雌激素的测量水平呈正相关。我们的研究结果说明了鱼类发育阶段对雌激素暴露效应的重要性，并证明了ERE-GFP斑马鱼对暴露于雌激素化学混合物的综合健康分析的效用。
A wide range of health effects in fish have been reported for exposure to wastewater treatment work (WwTW) effluents including feminized responses in males. Most of these exposure studies, however, have assessed acute health effects and chronic exposure effects are less well established. Using an Estrogen Responsive Element-Green Fluorescent Protein (ERE-GFP)-Casper transgenic zebrafish, we investigated chronic health effects and life stage sensitivities for exposure to an estrogenic WwTW effluent and the synthetic estrogen 17α-ethinylestradiol (EE2). Exposure to the WwTW effluent (at full strength;100%) and to 10 ng/L (nominal) EE2 delayed testis maturation in male fish but accelerated ovary development in females. Exposure to 50% and 100% effluent, and to 10 ng/L EE2, also resulted in skewed sex ratios in favor of females. Differing patterns of green fluorescent protein (GFP) expression, in terms of target tissues and developmental life stages occurred in the ERE-GFP- zebrafish chronically exposed to 100% effluent and reflected the estrogenic content of the effluent. gfp and vitellogenin (vtg) mRNA induction were positively correlated with measured levels of steroidal estrogens in the effluent throughout the study. Our findings illustrate the importance of a fish’s developmental stage for estrogen exposure effects and demonstrate the utility of the ERE-GFP zebrafish for integrative health analysis for exposure to estrogenic chemical mixtures.
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DOI：
10.1016/j.aquatox.2015.10.020
发表时间：
2015-12
期刊：
Aquatic toxicology (Amsterdam, Netherlands)
影响因子：
--
作者：
Chen J;Xiao Y;Gai Z;Li R;Zhu Z;Bai C;Tanguay RL;Xu X;Huang C;Dong Q
通讯作者：
Dong Q
影响因子：
5.6
作者：
De la Paz JF;Beiza N;Paredes-Zúñiga S;Hoare MS;Allende ML
通讯作者：
Allende ML
影响因子：
3.7
作者：
Fuzzen ML;Bragg LM;Tetreault GR;Bahamonde PA;Tanna RN;Bennett CJ;McMaster ME;Servos MR
通讯作者：
Servos MR
影响因子：
11.4
作者：
Coe, Tobias S.;Soeffker, Marta K.;Tyler, Charles R.
通讯作者：
Tyler, Charles R.
影响因子：
4.5
作者：
Baumann, Lisa;Holbech, Henrik;Braunbeck, Thomas
通讯作者：
Braunbeck, Thomas