Characterization of the nasopharyngeal microbiota in health and during rhinovirus challenge.
Characterization of the nasopharyngeal microbiota in health and during rhinovirus challenge.
复制标题
DOI：
10.1186/2049-2618-2-22
复制
发表时间：
2014
期刊：
影响因子：
15.5
通讯作者：
Sale MM
中科院分区：
文献类型：
--
作者：
Allen EK;Koeppel AF;Hendley JO;Turner SD;Winther B;Sale MM
鼻咽部的细菌群落在上呼吸道感染（URTI）中起着重要作用。我们的研究代表了在已知的、受控的病毒攻击期间对鼻咽部的首次调查。我们的目的是更好地了解病毒感染期间鼻咽微生物组的组成和动态。鼻病毒疾病是通过使用手指到鼻子或眼睛的自然传播途径在10名其他健康的年轻人中进行自我接种而引起的。在实验性鼻病毒接种之前、期间和之后的特定时间点采集鼻灌洗液样本（NLF）样本。通过扩增V1-V2高变区，然后使用454-FLX平台进行测序，对每份样本（10名受试者的N = 97）的细菌DNA进行16 S rRNA测序。这项对鼻咽微生物群的调查揭示了一个高度复杂的微生物生态系统。分类组成差异很大的主题和同一主题的时间点之间。我们还观察到，与感染个体相比，未感染个体的多样性显著更高。两个属-奈瑟氏菌属和丙酸杆菌属-感染和未感染个体之间存在显著差异。某些门，包括厚壁菌门，放线菌门，变形菌门，在所有样品中检测到。我们的研究结果揭示了健康和病毒挑战成人鼻咽微生物群的复杂性和多样性。虽然有些门是共同的所有样本，在感染和未感染的参与者之间检测到的多样性和选定的门的水平的差异。在更大的样本中进行更深入的物种水平的宏基因组测序是必要的。
The bacterial communities of the nasopharynx play an important role in upper respiratory tract infections (URTIs). Our study represents the first survey of the nasopharynx during a known, controlled viral challenge. We aimed to gain a better understanding of the composition and dynamics of the nasopharyngeal microbiome during viral infection. Rhinovirus illnesses were induced by self-inoculation using the finger to nose or eye natural transmission route in ten otherwise healthy young adults. Nasal lavage fluid samples (NLF) samples were collected at specific time points before, during, and following experimental rhinovirus inoculation. Bacterial DNA from each sample (N = 97 from 10 subjects) was subjected to 16S rRNA sequencing by amplifying the V1-V2 hypervariable region followed by sequencing using the 454-FLX platform. This survey of the nasopharyngeal microbiota revealed a highly complex microbial ecosystem. Taxonomic composition varied widely between subjects and between time points of the same subject. We also observed significantly higher diversity in not infected individuals compared to infected individuals. Two genera – Neisseria and Propionibacterium – differed significantly between infected and not infected individuals. Certain phyla, including Firmicutes, Actinobacteria, and Proteobacteria, were detected in all samples. Our results reveal the complex and diverse nature of the nasopharyngeal microbiota in both healthy and viral-challenged adults. Although some phyla were common to all samples, differences in levels of diversity and selected phyla were detected between infected and uninfected participants. Deeper, species-level metagenomic sequencing in a larger sample is warranted.
登录
查看更多内容
影响因子：
5.4
作者：
Avadhanula, V;Rodriguez, CA;Adderson, EE
通讯作者：
Adderson, EE
影响因子：
3.7
作者：
Bogaert D;Keijser B;Huse S;Rossen J;Veenhoven R;van Gils E;Bruin J;Montijn R;Bonten M;Sanders E
通讯作者：
Sanders E
影响因子：
3.6
作者：
Murphy, Timothy F.;Bakaletz, Lauren O.;Smeesters, Pierre R.
通讯作者：
Smeesters, Pierre R.
影响因子：
9.4
作者：
McCrea, Kirk W.;Xie, Jingping;Gilsdorf, Janet R.
通讯作者：
Gilsdorf, Janet R.
DOI：
10.1093/bioinformatics/btp636
发表时间：
2010-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Caporaso JG;Bittinger K;Bushman FD;DeSantis TZ;Andersen GL;Knight R
通讯作者：
Knight R