Structural relationship between the chicken DNA locus, proto-fps, and the transforming gene of Fujinami sarcoma virus, delta gag-fps.-猫眼课题宝

鸡 DNA 位点 proto-fps 与藤波肉瘤病毒转化基因 delta gag-fps 之间的结构关系。

DOI：

10.1016/0042-6822(83)90397-5

发表时间：

1983

期刊：

Virology

影响因子：

3.7

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

Lee,WH;Phares,W;Duesberg,PH

禽Fujinami肉瘤病毒含有一个混合转化基因(ΔGag-fps)，其5‘1.3kb的片段来自禽反转录病毒的Gag基因，3’2.8kb的片段来自于细胞原型。从鸡DNA文库中选择了一个在16kb的细胞DNA插入片段中携带fps序列的重组DNA克隆，称为lambda proto-fps克隆12，并与病毒基因进行了比较。抗内切酶原-fps DNA片段的定位和与克隆病毒DNA的杂交定位在插入片段的3‘端，约4.25kb。通过对DNA-RNA杂交物中常见的RNaseT1抗性寡核苷酸序列的比对，表明：(I)原fps与病毒fps共线，但被与病毒fps无关的1.75kb的零散序列打断；(Ii)在比较的9个内切酶位点中，原fps和病毒fps在同源位置共享一个PvuII、一个BamHI和可能的一个KpnI位点，并且它们在独特的位置上都有唯一的内切酶位点和共同的位点；(Iii)在与病毒fps重叠的5‘上游12kb的范围内，有proto-facksGag相关序列；和(Iv)原fps克隆12，与我们分离的其他几个克隆一样，在3‘端缺乏相当于3’10%至20%的viralfps。原核糖核酸和病毒DNA的8个内切酶位置图坐标也将病毒RNA的44个pSoligon核苷酸分成9个MAP片段。我们认为，口蹄疫病毒的癌基因不同于原Δ基因的多点突变，符合病毒基因的转换功能和细胞序列同源的正常功能。由于原fpsis与必要的病毒粒子基因无关，FSV的致癌基因一定是由细胞内的原fps通过罕见的、不合法的重组而来。

The avian Fujinami sarcoma virus (FSV) contains a hybrid transforming gene (Δgag-fps) with a 5′ 1.3-kb portion derived from the gag gene of avian retroviruses and a 3′ 2.8-kb portion (fps) derived from a cellular prototype. A lambda recombinant DNA clone carryingfpssequences within a 16-kb insert of cellular DNA, termed lambda proto-fpsclone 12, has been selected from a chicken DNA library for comparison with the viraloncgene. Mapping of endonuclease-resistant proto-fpsDNA fragments and hybridization with cloned viral DNA located FSV-related sequences at the 3′ end of the insert within a region of about 4.25 kb. Alignment of endonuclease-resistant proto-fpsand viral DNA fragments relative to common RNase T1-resistant oligonucleotide sequences of viral RNA, identified by fingerprinting DNA-RNA hybrids, indicated: (i) that proto-fpsis colinear with viralfpsbut is interrupted by 1.75 kb of scattered sequences unrelated to viralfps; (ii) that among the nine endonuclease sites compared, proto-fpsand viralfpsshare onePvuII, oneBamHI, and possibly aKpnI site at homologous locations, and that they each have unique endonuclease sites and common sites at unique locations; (iii) that within 12 kb upstream from the 5′ boundary of overlap with viralfps, proto-fpslacksgag-related sequences; and (iv) that proto-fpsclone 12, like several others isolated by us, lacks at the 3′ end an equivalent of the 3′ 10 to 20% of viralfps. The eight endonuclease site-map coordinates of proto-fpsand viral DNA also divided 44fpsoligonucleotides of viral RNA into 9 map segments. We conclude that theoncgene of FSV differs from proto-fpsin Δgagand in multiple point mutations, compatible with a transforming function for the viral gene and a normal function for the cellular sequence homolog. Since proto-fpsis unrelated to essential virion genes, theoncgene of FSV must have originated from cellular proto-fpsby rare, illegitimate recombination.