Retrieval of contextual memories increases activity-regulated cytoskeleton-associated protein in the amygdala and hippocampus.
Retrieval of contextual memories increases activity-regulated cytoskeleton-associated protein in the amygdala and hippocampus.
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DOI：
10.1007/s00429-012-0453-y
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发表时间：
2013-09
影响因子：
3.1
通讯作者：
Rademacher, David J.
中科院分区：
文献类型：
--
作者：
Figge, David A.;Rahman, IhteshamUr;Dougherty, Philip J.;Rademacher, David J.
关键词：
活性调节的细胞骨架相关蛋白（Arc）整合来自多个细胞内信号级联的信息，进而调节参与结构突触修饰的细胞骨架蛋白。本研究的目的是：(a)确定上下文记忆的检索是否会诱发海马和杏仁核神经元的Arc；(b)在超微结构水平上使用无偏立体学来量化接触Arc标记（Arc+）和未标记（Arc−）的脑区突触后结构的突触，其中Arc整合密度（ID）的数量与安非他明条件位置偏好（AMPH CPP）的程度密切相关。背景记忆的检索增加了海马齿状回、海马角区（CA）1和CA3区以及杏仁核基底外侧、外侧和中央核的Arc ID，但没有增加初级听觉皮层（一个控制区）的Arc ID。研究人员对杏仁核基底外侧核（BLA）的Arc+和Arc−突触进行了立体学定量分析，因为在BLA中观察到Arc ID的数量与AMPH CPP的数量之间存在最强的关系。背景记忆的检索增加了与BLA神经元的Arc+棘和树突接触的非对称突触（假设为兴奋性）、与BLA神经元的Arc+树突接触的对称突触（假设为抑制性或调节性）和与Arc+突触后结构接触的多突触钮扣的数量和密度。因此，背景记忆的检索增加了杏仁核和海马体中的Arc，这一效应对于接近与药物相关的环境的行为可能是重要的。
Activity-regulated cytoskeleton-associated protein (Arc) integrates information from multiple intracellular signaling cascades and, in turn, regulates cytoskeletal proteins involved in structural synaptic modifications. The purposes of the present study were: (a) to determine if the retrieval of contextual memories would induce Arc in hippocampal and amygdalar neurons; and (b) use unbiased stereology at the ultrastructural level to quantify synapses contacting Arc-labeled (Arc+) and unlabeled (Arc−) postsynaptic structures in brain regions in which the amount of Arc integrated density (ID) correlated strongly with the degree of amphetamine conditioned place preference (AMPH CPP). The retrieval of contextual memories increased the Arc ID in the dentate gyrus, cornu ammonis (CA)1, and CA3 fields of the hippocampus and the basolateral, lateral, and central nuclei of the amygdala but not the primary auditory cortex, a control region. Stereological quantification of Arc+ and Arc− synapses in the basolateral nucleus of the amygdala (BLA) was undertaken because the strongest relationship between the amount of Arc ID and AMPH CPP was observed in the BLA. The retrieval of contextual memories increased the number and density of asymmetric (presumed excitatory) synapses contacting Arc+ spines and dendrites of BLA neurons, symmetric (presumed inhibitory or modulatory) synapses contacting Arc+ dendrites of BLA neurons, and multisynaptic boutons contacting Arc+ postsynaptic structures. Thus, the retrieval of contextual memories increases Arc in the amygdala and hippocampus, an effect that could be important for approach behavior to a drug-associated context.
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影响因子：
5.3
作者：
Bothwell, S;Meredith, GE;Farrell, M
通讯作者：
Farrell, M
影响因子：
3.6
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作者：
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影响因子：
3.3
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影响因子：
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作者：
CALVERLEY, RKS;BEDI, KS;JONES, DG
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