Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins.
Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins.
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在缺乏TRAP家族粘附素的情况下，疟原虫子孢子的滑行运动有限。
DOI：
10.1186/s12936-021-03960-3
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发表时间：
2021-10-30
期刊：
影响因子：
3
通讯作者：
Frischknecht F
中科院分区：
文献类型：
--
作者：
Beyer K;Kracht S;Kehrer J;Singer M;Klug D;Frischknecht F
疟原虫子孢子是由蚊子传播给脊椎动物宿主的引起疟疾的寄生虫的高度运动形式。子孢子需要进入并穿过几个细胞和组织屏障，为此它们采用一组表面蛋白。这些蛋白质中的三种是血小板反应蛋白相关匿名蛋白（TRAP）家族的成员。在这里，这些粘附蛋白的潜在的相加，协同或拮抗作用进行了研究。使用阳性-阴性选择产生四种缺乏TRAP家族粘附素TRAP、TLP和TREP中的两种或全部三种的转基因伯氏疟原虫寄生虫系。研究了寄生虫系在玻璃上附着和移动的能力、从卵囊中逸出的能力以及进入蚊子唾液腺的能力。另外还研究了一种菌株感染小鼠的能力。TRAP单基因缺失的主要表型主导其他基因缺失表型。所有的寄生虫品系，包括缺乏所有三种蛋白质的品系，都能够进行某种形式的活跃运动，即使是非生产性运动。个别TRAP家族粘附素似乎在运动和感染过程中发挥功能不同的作用。其他蛋白质必须有助于底物粘附和滑动运动。在线版本包含补充材料，可通过10.1186/s12936-021-03960-3获得。
Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated. Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive–negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocysts and their capacity to enter mosquito salivary glands. One strain was in addition interrogated for its capacity to infect mice. The major phenotype of the TRAP single gene deletion dominates additional gene deletion phenotypes. All parasite lines including the one lacking all three proteins were able to conduct some form of active, if unproductive movement. The individual TRAP-family adhesins appear to play functionally distinct roles during motility and infection. Other proteins must contribute to substrate adhesion and gliding motility. The online version contains supplementary material available at 10.1186/s12936-021-03960-3.
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3
作者：
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通讯作者：
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通讯作者：
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