Detection of dengue virus serotypes 1, 2 and 3 in selected regions of Kenya: 2011-2014.
Detection of dengue virus serotypes 1, 2 and 3 in selected regions of Kenya: 2011-2014.
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DOI：
10.1186/s12985-016-0641-0
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发表时间：
2016-11-04
期刊：
影响因子：
4.8
通讯作者：
Sang R
中科院分区：
文献类型：
--
作者：
Konongoi L;Ofula V;Nyunja A;Owaka S;Koka H;Makio A;Koskei E;Eyase F;Langat D;Schoepp RJ;Rossi CA;Njeru I;Coldren R;Sang R
登革热是一种由蚊子传播的疾病，在热带和亚热带国家与不同严重程度的疾病有关。登革热病例继续被更频繁地发现，其地理范围继续扩大。我们报告了自1982年以来肯尼亚部分地区有记录的最大规模的实验室确认的登革热病毒传播。从2011年9月至2014年12月，从内罗毕、肯尼亚北部和沿海的医院收到了868份发烧患者的样本。采用免疫球蛋白M酶联免疫吸附试验(IgM-EL ISA)检测登革热、黄热病、西尼罗河病毒和寨卡病毒的IgM抗体。用黄病毒家族、黄热病、西尼罗河病毒、共同型和血清型登革热的逆转录聚合酶链式反应(RT-PCR)检测急性虫媒病毒感染，确定感染的血清型。对检测到的三个登革热血清型中每一个的聚合酶链式反应阳性样本的代表性样本进行了测序，以确认各种登革热血清型的传播。40.00%(345/868)的标本经IgM-EL ISA或RT-PCR检测为登革热阳性(14.6%)或(25.1%)。用血清型特异性RT-PCR和测序方法检测了3个登革热血清型1-3(DENV1-3)，它们的数量在不同年份和不同地区有所不同。2011-2014年间检出的主要血清型为DENV1型，占全部检出血清型的44.0%(96/218)，其次为DENV2型，占38.5%(84/218)，其次为DENV3型，占17.4%(38/218)。在测试的任何样本中都没有检测到黄热病、西尼罗河病毒和寨卡病毒。2011-2014年，在肯尼亚北部和沿海地区发现了1型、2型和3型血清。这证实了在肯尼亚部分地区发生了登革热病例和活跃的传播。这些结果记录了三种流行的血清型，并突出表明需要建立积极的登革热监测，以持续发现病例、传播血清型并确定国家和区域的登革热疾病负担。
Dengue fever, a mosquito-borne disease, is associated with illness of varying severity in countries in the tropics and sub tropics. Dengue cases continue to be detected more frequently and its geographic range continues to expand. We report the largest documented laboratory confirmed circulation of dengue virus in parts of Kenya since 1982. From September 2011 to December 2014, 868 samples from febrile patients were received from hospitals in Nairobi, northern and coastal Kenya. The immunoglobulin M enzyme linked immunosorbent assay (IgM ELISA) was used to test for the presence of IgM antibodies against dengue, yellow fever, West Nile and Zika. Reverse transcription polymerase chain reaction (RT-PCR) utilizing flavivirus family, yellow fever, West Nile, consensus and sero type dengue primers were used to detect acute arbovirus infections and determine the infecting serotypes. Representative samples of PCR positive samples for each of the three dengue serotypes detected were sequenced to confirm circulation of the various dengue serotypes. Forty percent (345/868) of the samples tested positive for dengue by either IgM ELISA (14.6 %) or by RT-PCR (25.1 %). Three dengue serotypes 1–3 (DENV1-3) were detected by serotype specific RT-PCR and sequencing with their numbers varying from year to year and by region. The overall predominant serotype detected from 2011–2014 was DENV1 accounting for 44 % (96/218) of all the serotypes detected, followed by DENV2 accounting for 38.5 % (84/218) and then DENV3 which accounted for 17.4 % (38/218). Yellow fever, West Nile and Zika was not detected in any of the samples tested. From 2011–2014 serotypes 1, 2 and 3 were detected in the Northern and Coastal parts of Kenya. This confirmed the occurrence of cases and active circulation of dengue in parts of Kenya. These results have documented three circulating serotypes and highlight the need for the establishment of active dengue surveillance to continuously detect cases, circulating serotypes, and determine dengue fever disease burden in the country and region.
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影响因子：
11.8
作者：
Leroy EM;Nkoghe D;Ollomo B;Nze-Nkogue C;Becquart P;Grard G;Pourrut X;Charrel R;Moureau G;Ndjoyi-Mbiguino A;De-Lamballerie X
通讯作者：
De-Lamballerie X
DOI：
10.4269/ajtmh.2001.65.180
发表时间：
2001-09-01
影响因子：
3.3
作者：
Halstead, SB;Streit, TG;Watts, DM
通讯作者：
Watts, DM
影响因子：
4.8
作者：
Mease, Luke E.;Coldren, Rodney L.;Adungo, Nicholas
通讯作者：
Adungo, Nicholas
从沙特阿拉伯吉达分离的1型登革热1型病毒的完整基因组测序和系统发育分析。
影响因子：
4.8
作者：
Azhar EI;Hashem AM;El-Kafrawy SA;Abol-Ela S;Abd-Alla AM;Sohrab SS;Farraj SA;Othman NA;Ben-Helaby HG;Ashshi A;Madani TA;Jamjoom G
通讯作者：
Jamjoom G
DOI：
10.1016/j.cimid.2004.03.013
发表时间：
2004-09-01
影响因子：
2
作者：
Gubler, DJ
通讯作者：
Gubler, DJ