Comparative transcriptome analysis reveals different molecular mechanisms of Bacillus coagulans 2-6 response to sodium lactate and calcium lactate during lactic acid production.
Comparative transcriptome analysis reveals different molecular mechanisms of Bacillus coagulans 2-6 response to sodium lactate and calcium lactate during lactic acid production.
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DOI：
10.1371/journal.pone.0124316
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Qin J;Wang X;Wang L;Zhu B;Zhang X;Yao Q;Xu P
在发酵过程中加入碳酸钙或氢氧化钠可以提高乳酸的产量。而凝结芽孢杆菌2-6在乳酸钙积累时可产生180 g/L以上的L-乳酸，而在乳酸钠形成时可产生120 g/L以下的L-乳酸。凝结芽孢杆菌对乳酸钙和乳酸钠反应的分子机制尚不清楚。本研究采用基于高通量RNA测序的比较转录组学方法，研究非胁迫、乳酸钠胁迫和乳酸钙胁迫条件下培养的凝血芽孢杆菌2-6的基因表达变化。基因表达谱分析发现712个和1213个基因在乳酸钙胁迫和乳酸钠胁迫下分别受到显著调控。对差异表达基因进行基因本体赋值。KEGG途径富集分析显示，乳酸钙胁迫显著影响了“atp结合盒转运体”，乳酸钠胁迫显著影响了“氨基糖和核苷酸糖代谢”。乳酸钙胁迫对乳酸发酵的影响小于乳酸钠胁迫。乳酸钠胁迫对“糖酵解/糖异生”基因的表达有负向影响，而对“柠檬酸循环”基因的表达有正向影响。乳酸钙胁迫对“糖酵解/糖异生”基因的表达有正向影响，对“柠檬酸循环”基因的表达影响较小。因此，我们的研究结果为研究凝结芽孢杆菌对不同乳酸胁迫的反应提供了新的见解。值得注意的是，我们的RNA-seq数据集构成了一个强大的数据库，用于研究未来乳酸胁迫诱导的基因功能，并确定基因工程的潜在目标，以进一步提高B.凝固菌的l -乳酸产量。
Lactate production is enhanced by adding calcium carbonate or sodium hydroxide during fermentation. However, Bacillus coagulans 2-6 can produce more than 180 g/L L-lactic acid when calcium lactate is accumulated, but less than 120 g/L L-lactic acid when sodium lactate is formed. The molecular mechanisms by which B. coagulans responds to calcium lactate and sodium lactate remain unclear. In this study, comparative transcriptomic methods based on high-throughput RNA sequencing were applied to study gene expression changes in B. coagulans 2-6 cultured in non-stress, sodium lactate stress and calcium lactate stress conditions. Gene expression profiling identified 712 and 1213 significantly regulated genes in response to calcium lactate stress and sodium lactate stress, respectively. Gene ontology assignments of the differentially expressed genes were performed. KEGG pathway enrichment analysis revealed that ‘ATP-binding cassette transporters’ were significantly affected by calcium lactate stress, and ‘amino sugar and nucleotide sugar metabolism’ was significantly affected by sodium lactate stress. It was also found that lactate fermentation was less affected by calcium lactate stress than by sodium lactate stress. Sodium lactate stress had negative effect on the expression of ‘glycolysis/gluconeogenesis’ genes but positive effect on the expression of ‘citrate cycle (TCA cycle)’ genes. However, calcium lactate stress had positive influence on the expression of ‘glycolysis/gluconeogenesis’ genes and had minor influence on ‘citrate cycle (TCA cycle)’ genes. Thus, our findings offer new insights into the responses of B. coagulans to different lactate stresses. Notably, our RNA-seq dataset constitute a robust database for investigating the functions of genes induced by lactate stress in the future and identify potential targets for genetic engineering to further improve L-lactic acid production by B. coagulans.
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影响因子：
3.6
作者：
Greven, Corina U.;Asherson, Philip;Plomin, Robert
通讯作者：
Plomin, Robert
影响因子：
25.8
作者：
Chang, Zheng;Lichtenstein, Paul;Larsson, Henrik
通讯作者：
Larsson, Henrik
影响因子：
3
作者：
Boker S;Neale M;Maes H;Wilde M;Spiegel M;Brick T;Spies J;Estabrook R;Kenny S;Bates T;Mehta P;Fox J
通讯作者：
Fox J
DOI：
10.1111/j.1741-1130.2011.00281.x
发表时间：
2011-03-01
影响因子：
1.7
作者：
Firkowska-Mankiewicz, Anna
通讯作者：
Firkowska-Mankiewicz, Anna
影响因子：
4.8
作者：
Biederman J;Petty CR;Clarke A;Lomedico A;Faraone SV
通讯作者：
Faraone SV