Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves.
Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves.
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断奶前犊牛显性肠细胞虫 bieneusi 基因型的遗传多样性
DOI：
10.1186/s13071-018-2768-x
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发表时间：
2018-03-12
影响因子：
3.2
通讯作者：
Xiao L
中科院分区：
文献类型：
--
作者：
Tang C;Cai M;Wang L;Guo Y;Li N;Feng Y;Xiao L
背景：牛常感染微孢子虫寄生虫比内氏肠孢子虫。在核糖体内转录间隔区(ITS)座位上对这些动物中的毕氏艾美耳球虫进行了序列分析，确定I、J和BEB4为优势基因型。然而，目前对奶牛感染毕氏艾美耳球虫的研究主要集中在感染率和基因分布上。本研究旨在研究中国上海地区早期断奶犊牛中比埃希氏肠杆菌优势基因的遗传多样性。方法：以养殖场为材料，通过ITS序列分析和多位点序列分型(MLST)，鉴定出比氏肠球虫的基因分型和亚型。结果：总感染率为26.5%(214/809)，范围为12.6%(5号场)~38.5%(4号场)。早期感染率随年龄增长而增加，6周龄时感染率最高(43.0%；43/100)。共检出J(n=145，67.8%)、BEB4(n=59，27.6%)、CHN4(n=4，1.9%)和CHN15(n=1，0.5%)4种基因型，前两种属于第2组，后两种属于第1组。在两个显性ITS基因型中共发现10个多位基因座(MLG)，包括J型的MLG-J1~MLG-J8和BEB4型的MLG-B1~MLG-B2。在1、2、5场分别检测到MLG-B1和MLG-B2，而在3、4场分别检测到MLG-J1~MLG-J5和MLG-J6~MLG-J8。结论：上海、中国和MLST牛群中优势基因J和BEB4存在广泛的遗传异质性，可用于牛的分子流行病学研究。
Background:Cattle are commonly infected with the microsporidian parasite Enterocytozoon bieneusi. Sequence characterization of E. bieneusi in these animals at the ribosomal internal transcribed spacer (ITS) locus had identified I, J and BEB4 as the dominant genotypes. However, current studies on E. bieneusi in dairy cattle are mostly on infection rates and genotype distribution. This study aims to examine the intragenotypic diversity within dominant E. bieneusi genotypes in pre-weaned dairy calves in Shanghai, China.Methods:Enterocytozoon bieneusi genotypes and subtypes were identified by PCR sequence analysis of ITS and multilocus sequence typing (MLST), based on material from farms. Chi-square test was used to examine differences in E. bieneusi infection rates between farms or age groups.Results:The overall infection rate of E. bieneusi was 26.5% (214/809), ranging from 12.6% (Farm 5) to 38.5% (Farm 4). Infection rates increased with age during early life, with the peak infection rate (43.0%; 43/100) occurring at six weeks. Four genotypes were present, including J (n = 145, 67.8%), BEB4 (n = 59, 27.6%), CHN4 (n = 4, 1.9%) and CHN15 (n = 1, 0.5%), with the former two belonging to Group 2 and the latter two belonging to Group 1. Differences were detected in the distribution of the dominant genotypes J and BEB4 among five study farms. Altogether, 10 multilocus genotypes (MLGs) were identified in the two dominant ITS genotypes, including MLG-J1 to MLG-J8 of genotype J and MLG-B1 to MLG-B2 of genotype BEB4. MLG-B1 and MLG-B2 were recovered in Farms 1, 2 and 5, whereas MLG-J1 to MLG-J5 and MLG-J6 to MLG-J8 were found in Farms 3 and 4, respectively.Conclusions:There is extensive genetic heterogeneity within the dominant E. bieneusi genotypes J and BEB4 in dairy calves in Shanghai, China, and MLST should be used in molecular epidemiological studies of E. bieneusi in cattle.
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