Bacterial attraction and quorum sensing inhibition in Caenorhabditis elegans exudates.
Bacterial attraction and quorum sensing inhibition in Caenorhabditis elegans exudates.
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DOI：
10.1007/s10886-009-9670-0
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发表时间：
2009-08
影响因子：
2.3
通讯作者：
Edison, Arthur S.
中科院分区：
文献类型：
--
作者：
Kaplan, Fatma;Badri, Dayakar V.;Zachariah, Cherian;Ajredini, Ramadan;Sandoval, Francisco J.;Roje, Sanja;Levine, Lanfang H.;Zhang, Fengli;Robinette, Steven L.;Alborn, Hans T.;Zhao, Wei;Stadler, Michael;Nimalendran, Rathika;Dossey, Aaron T.;Brueschweiler, Rafael;Vivanco, Jorge M.;Edison, Arthur S.
关键词：
秀丽隐杆线虫是一种食菌线虫，生活在复杂的腐烂水果、土壤和堆肥环境中，化学相互作用是交配、监测种群密度、识别食物、避免病原微生物和其他基本生态功能所必需的。尽管它是生物学中研究得最好的模式生物之一，但人们对C。线虫用来与环境发生化学反应或作为防御。C.使用几种分析方法分析了秀丽线虫的分泌物，发现其含有36种常见的代谢物，包括有机酸、氨基酸和糖，所有这些代谢物的丰度都相对较高。此外，分泌物中氨基酸的浓度依赖于发育阶段。梭用恶臭假单胞菌（Pseudomonasputida，KT 2440）和铜绿假单胞菌（Pseudomonasaeruginosa，PAO 1）对线虫分泌物进行趋化性试验。elegans和E.大肠杆菌（OP 50），作为对照测试的非运动细菌。梭秀丽隐杆线虫分泌物吸引两种假单胞菌属物种，但对铜绿假单胞菌没有可检测的抗菌活性。结果表明，在成虫期和幼成虫期的分泌物中，C.线虫分泌物抑制了基于LuxR细菌群体感应（QS）系统的报告系统中的群体感应，该系统调节费氏弧菌中的细菌毒力和其他因子。我们能够对QS抑制和细菌趋化活性进行分级，证明这些活性在化学上是不同的。结果表明，C.线虫能够吸引细菌食物，并具有通过抑制特定QS系统来部分调节细菌病原体毒力的潜力。
Caenorhabditis elegans, a bacterivorous nematode, lives in complex rotting fruit, soil, and compost environments, and chemical interactions are required for mating, monitoring population density, recognition of food, avoidance of pathogenic microbes, and other essential ecological functions. Despite being one of the best-studied model organisms in biology, relatively little is known about the signals that C. elegans uses to chemically interact with its environment or as defense. C. elegans exudates were analyzed using several analytical methods and found to contain 36 common metabolites including organic acids, amino acids and sugars, all in relatively high abundance. Furthermore, the concentrations of amino acids in the exudates were dependent on developmental stage. The C. elegans exudates were tested for bacterial chemotaxis using Pseudomonas putida (KT2440), a plant growth promoting rhizobacterium, Pseudomonas aeruginosa (PAO1), a soil bacterium pathogenic to C. elegans, and E. coli (OP50), a non-motile bacterium tested as a control. The C. elegans exudates attracted the two Psuedomonas species, but had no detectable antibacterial activity against P. aeruginosa. To our surprise, the exudates of young adult and adult life stages of C. elegans exudates inhibited quorum sensing in the reporter system based on the LuxR bacterial quorum sensing (QS) system, which regulates bacterial virulence and other factors in Vibrio fischeri. We were able to fractionate the QS inhibition and bacterial chemotaxis activities, demonstrating that these activities are chemically distinct. Our results demonstrate that C. elegans can attract its bacterial food and has the potential of partially regulating the virulence of bacterial pathogens by inhibiting specific QS systems.
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影响因子：
3.2
作者：
ADLER, J;HAZELBAUER, GL;DAHL, MM
通讯作者：
DAHL, MM
影响因子：
64.8
作者：
Bais, HP;Prithiviraj, B;Vivanco, JM
通讯作者：
Vivanco, JM
影响因子：
46.9
作者：
Fiehn, O;Kopka, J;Willmitzer, L
通讯作者：
Willmitzer, L
影响因子：
4.2
作者：
Fothergill JL;Panagea S;Hart CA;Walshaw MJ;Pitt TL;Winstanley C
通讯作者：
Winstanley C
DOI：
10.1073/pnas.96.26.15202
发表时间：
1999-12-21
影响因子：
11.1
作者：
Darby, C;Cosma, CL;Manoil, C
通讯作者：
Manoil, C