The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.
The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.
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DOI：
10.1083/jcb.111.2.309
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发表时间：
1990-08
期刊：
影响因子：
--
通讯作者：
Dunst RW
中科院分区：
文献类型：
--
作者：
Hopper AK;Traglia HM;Dunst RW
酵母RNA1基因是RNA加工和RNA核运输所必需的。该位点的 rna1-1 突变导致前 tRNA 剪接、初级前 rRNA 转录物的加工、mRNA 的产生以及 RNA 从细胞核到细胞质的输出出现缺陷。为了了解该基因产物如何对这些过程产生多效性影响，我们试图确定 RNA1 蛋白的细胞内位置。通过间接免疫荧光定位和细胞器分级分离确定，RNA1 抗原仅或主要存在于细胞质中。只有一小部分内源蛋白可以定位于细胞核并在细胞核中发挥作用。此外，RNA1抗原在应激条件下的定位并没有不同。这些发现表明RNA1蛋白不直接参与RNA加工，但可能修饰核蛋白或以其他方式将信号从细胞质传递到细胞核或在维持细胞核的完整性中发挥作用。
The yeast RNA1 gene is required for RNA processing and nuclear transport of RNA. The rna1-1 mutation of this locus causes defects in pre-tRNA splicing, processing of the primary pre-rRNA transcript, production of mRNA and export of RNA from the nucleus to the cytosol. To understand how this gene product can pleiotropically affect these processes, we sought to determine the intracellular location of the RNA1 protein. As determined by indirect immunofluorescence localization and organelle fractionation, the RNA1 antigen is found exclusively or primarily in the cytoplasm. Only a tiny fraction of the endogenous protein could be localized to and functional in the nucleus. Furthermore, the RNA1 antigen does not localize differently under stress conditions. These findings suggest that the RNA1 protein is not directly involved in RNA processing but may modify nuclear proteins or otherwise transmit a signal from the cytosol to the nucleus or play a role in maintaining the integrity of the nucleus.
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影响因子：
64.5
作者：
BAEUERLE, PA;BALTIMORE, D
通讯作者：
BALTIMORE, D
影响因子：
3.5
作者：
BOTSTEIN, D;FALCO, SC;DAVIS, RW
通讯作者：
DAVIS, RW
影响因子：
5.3
作者：
BJORN, SP;SOLTYK, A;FRIESEN, JD
通讯作者：
FRIESEN, JD
影响因子：
10.5
作者：
COUTO, JR;TAMM, J;GUTHRIE, C
通讯作者：
GUTHRIE, C
DOI：
10.1073/pnas.81.13.3983
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DOUGLAS, MG;GELLER, BL;EMR, SD
通讯作者：
EMR, SD