BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
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DOI：
10.1371/journal.pone.0117566
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ohnishi K
中科院分区：
文献类型：
--
作者：
Funakoshi S;Shimizu T;Numata O;Ato M;Melchers F;Ohnishi K
记忆B细胞（MBC）和长寿浆细胞（LLPC）负责免疫“记忆”，可持续多年。LLPC在骨髓中的长期生存生态位已得到很好的表征;然而，MBC的相应生态位尚不清楚。BILL-钙粘蛋白/钙粘蛋白-17（CDH 17）是钙粘蛋白超家族中唯一以时空调节的方式在小鼠B淋巴细胞上表达的成员。在这里，我们发现，一半的MBC在发育后期重新表达CDH 17。与野生型小鼠（WT）相比，CDH 17-/-小鼠中高亲和力抗原特异性血清抗体的维持受损，抗原特异性MBC的数量减少。此外，在CDH 17-/-小鼠中消除了对第二抗原的特异性应答，而第一抗体应答与WT小鼠中的那些相同。细胞周期分析显示，与CDH 17 + MBCs相比，CDH 17- MBCs的增殖下降。此外，我们鉴定了脾基质细胞的亚群，MAdCAM-1+血内皮细胞（BEC），其为CDH 17+。总之，这些结果表明，CDH 17在MBC的长期存活中起作用，推测是通过至少部分地包含脾脏中的BEC的“MBC生态位”。
Memory B cells (MBCs) and long-lived plasma cells (LLPCs) are responsible for immunological “memory”, which can last for many years. The long-term survival niche for LLPCs in the bone marrow is well characterized; however, the corresponding niche for MBCs is unclear. BILL-cadherin/cadherin-17 (CDH17) is the only member of the cadherin superfamily that is expressed on mouse B lymphocytes in a spatiotemporally regulated manner. Here, we show that half of all MBCs regain expression of CDH17 during the later stage of development. The maintenance of high affinity antigen-specific serum antibodies was impaired in CDH17-/- mice and the number of antigen-specific MBCs was reduced as compared to wild-type mice (WT). Also, specific responses to secondary antigens were ablated in CDH17-/- mice, whereas primary antibody responses were the same as those in WT mice. Cell cycle analysis revealed a decline in the proliferation of CDH17- MBCs as compared to CDH17+ MBCs. In addition, we identified a subpopulation of splenic stromal cells, MAdCAM-1+ blood endothelial cells (BEC), which was CDH17+. Taken together, these results suggest that CDH17 plays a role in the long-term survival of MBCs, presumably via an “MBC niche” comprising, at least in part, BEC in the spleen.
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影响因子：
64.8
作者：
CEPEK, KL;SHAW, SK;BRENNER, MB
通讯作者：
BRENNER, MB
影响因子：
2.5
作者：
Angres, B;Kim, L;Tauber, R
通讯作者：
Tauber, R
DOI：
10.1084/jem.20062571
发表时间：
2007-09-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Anderson SM;Tomayko MM;Ahuja A;Haberman AM;Shlomchik MJ
通讯作者：
Shlomchik MJ
影响因子：
5.4
作者：
LIU, YJ;OLDFIELD, S;MACLENNAN, ICM
通讯作者：
MACLENNAN, ICM
影响因子：
30.5
作者：
Dogan, Ismail;Bertocci, Barbara;Weill, Jean-Claude
通讯作者：
Weill, Jean-Claude