Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model.
Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model.
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DOI：
10.1038/msb4100190
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发表时间：
2008
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
作者：
暴露于副干酪乳杆菌或鼠李糖乳杆菌益生菌的转基因代谢效应已经在人源化的扩展基因组小鼠（人类婴儿菌群定植的无菌小鼠）中进行了测量和绘制。对不同代谢区室（包括生物体液、组织和盲肠短链脂肪酸（SCFAs））与微生物种群调节相关的区室波动进行统计分析，产生了一种新的自上而下的系统生物学观点，可以研究宿主对益生菌干预的反应。暴露在益生菌环境中会改变微生物组，导致肝脏脂质代谢改变，同时降低血浆脂蛋白水平，明显刺激糖酵解。益生菌治疗也改变了多种途径的结果，包括氨基酸代谢、甲胺和scfa。层次主成分分析的新应用允许可视化多室转基因代谢相互作用，也可以在室和途径水平上解决。这些综合系统研究表明，代谢谱分析作为一种自上而下的系统生物学驱动程序，可以研究益生菌作用的机制基础，以及与膳食补充益生菌相关的肠道微生物活性的治疗性监测。
The transgenomic metabolic effects of exposure to either Lactobacillus paracasei or Lactobacillus rhamnosus probiotics have been measured and mapped in humanized extended genome mice (germ-free mice colonized with human baby flora). Statistical analysis of the compartmental fluctuations in diverse metabolic compartments, including biofluids, tissue and cecal short-chain fatty acids (SCFAs) in relation to microbial population modulation generated a novel top-down systems biology view of the host response to probiotic intervention. Probiotic exposure exerted microbiome modification and resulted in altered hepatic lipid metabolism coupled with lowered plasma lipoprotein levels and apparent stimulated glycolysis. Probiotic treatments also altered a diverse range of pathways outcomes, including amino-acid metabolism, methylamines and SCFAs. The novel application of hierarchical-principal component analysis allowed visualization of multicompartmental transgenomic metabolic interactions that could also be resolved at the compartment and pathway level. These integrated system investigations demonstrate the potential of metabolic profiling as a top-down systems biology driver for investigating the mechanistic basis of probiotic action and the therapeutic surveillance of the gut microbial activity related to dietary supplementation of probiotics.
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影响因子：
4.4
作者：
Corcoran, BM;Stanton, C;Ross, RP
通讯作者：
Ross, RP
影响因子：
4.4
作者：
ALLISON, C;MACFARLANE, GT
通讯作者：
MACFARLANE, GT
影响因子：
4.4
作者：
Das, UN
通讯作者：
Das, UN
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1097/00005176-200010000-00024
发表时间：
2000-10-01
影响因子：
2.9
作者：
Gupta, P;Andrew, H;Guandalini, S
通讯作者：
Guandalini, S