Trilayered tissue construct mimicking the orientations of three layers of a native heart valve leaflet.
Trilayered tissue construct mimicking the orientations of three layers of a native heart valve leaflet.
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DOI：
10.1007/s00441-020-03241-6
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发表时间：
2020-11
影响因子：
3.6
通讯作者：
Lerman A
中科院分区：
文献类型：
--
作者：
Jana S;Lerman A
组织工程化心脏瓣膜可以替代心脏瓣膜置换术中的人工瓣膜;然而，它们在持久功能方面并不完全有效，因为工程化瓣膜中的瓣叶不具有天然三层瓣叶结构。在此之前，我们开发了平坦的三层定向纳米纤维（TN）支架，模仿天然心脏瓣膜小叶的三层结构和取向。体内组织工程-一种实用的再生医学技术-可用于开发自体心脏瓣膜。因此，在这项研究中，我们使用我们的平面，三层，定向纳米纤维支架开发三层组织结构，通过在大鼠模型中的体内组织工程与天然小叶方向。经过两个月的体内组织工程，发现浸润的细胞及其沉积的胶原纤维在周向层和径向层中排列，并在支架的随机层中随机取向;即，开发了三层组织构建体（TTC）。由于组织工程中支架纤维的影响，TTCs的拉伸性能高于对照组织构建物（没有任何支架）的拉伸性能。在TTC中观察到存在于天然瓣叶中的不同细胞外基质组分-胶原蛋白、糖胺聚糖和弹性蛋白。TTC中细胞的基因表达表明组织构建体处于生长阶段。组织结构中没有钙化迹象。因此，用平坦的TN支架开发的TTC意味着可以开发可以用作天然小叶的自体小叶形状的三层组织构建体。
Tissue-engineered heart valves can be an alternative to prosthetic valves in heart valve replacement; however, they are not fully efficient in terms of long-lasting functionality as leaflets in engineered valves do not possess the native trilayered leaflet structure. Previously, we developed flat, trilayered, oriented nanofibrous (TN) scaffolds mimicking the trilayered structure and orientation of native heart valve leaflets. In-vivo tissue engineering – a practical regenerative medicine technology – can be used to develop autologous heart valves. Thus, in this study, we used our flat, trilayered, oriented nanofibrous scaffolds to develop trilayered tissue structures with native leaflet orientations through in-vivo tissue engineering in a rat model. After two months of in-vivo tissue engineering, infiltrated cells and their deposited collagen fibrils were found aligned in the circumferential and radial layers, and randomly oriented in the random layer of the scaffolds; i.e., trilayered tissue constructs (TTCs) were developed. The tensile properties of the TTCs were higher than that of the control tissue constructs (without any scaffolds) due to the influence of fibers of the scaffolds in tissue engineering. Different extracellular matrix components – collagen, glycosaminoglycans and elastin that exist in native leaflets were observed in the TTCs. Gene expression of the cells in the TTCs indicated that the tissue constructs were in the growing stage. There was no sign of calcification in the tissue constructs. Thus, the TTCs developed with the flat TN scaffolds signify that autologous leaflet-shaped, trilayered tissue constructs that can function as native leaflets can be developed.
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骨骼肌肉组织工程的各向异性材料。
DOI：
10.1002/adma.201600240
发表时间：
2016-12
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Jana S;Levengood SK;Zhang M
通讯作者：
Zhang M
影响因子：
7
作者：
Jana, Soumen;Zhang, Miqin
通讯作者：
Zhang, Miqin
影响因子：
9.5
作者：
Jana, Soumen;Lerman, Amir;Simarit, Robert D.
通讯作者：
Simarit, Robert D.
影响因子：
9.5
作者：
Jana, Soumen;Cooper, Ashleigh;Zhang, Miqin
通讯作者：
Zhang, Miqin
影响因子：
14
作者：
Masoumi, Nafiseh;Annabi, Nasim;Assmann, Alexander;Larson, Benjamin L;Hjortnaes, Jesper;Alemdar, Neslihan;Kharaziha, Mahshid;Manning, Keefe B;Mayer, John E Jr;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali