Antigenic analysis of feline coronaviruses with monoclonal antibodies (MAbs): preparation of MAbs which discriminate between FIPV strain 79-1146 and FECV strain 79-1683.
Antigenic analysis of feline coronaviruses with monoclonal antibodies (MAbs): preparation of MAbs which discriminate between FIPV strain 79-1146 and FECV strain 79-1683.
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DOI：
10.1016/0378-1135(91)90096-x
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发表时间：
1991-06
影响因子：
3.3
通讯作者：
Koyama H
中科院分区：
文献类型：
--
作者：
Hohdatsu T;Sasamoto T;Okada S;Koyama H
我们制备了 31 种针对 FIPV 毒株 79-1146 或 FECV 毒株 79-1683 的单克隆抗体 (MAb)，并通过间接荧光抗体测定 (IFA) 测试了它们与各种冠状病毒的反应性。 16 种单克隆抗体与所有 11 种猫冠状病毒株发生反应，也与犬冠状病毒 (CCV) 和传染性胃肠炎病毒 (TGEV) 发生反应。其中许多多肽的特异性是对病毒跨膜（E1）蛋白的识别。我们成功获得了不与八株 FIPV I 型病毒（显示细胞相关生长）反应但与 FIPV II 型（79-1146，KU-1）和/或 FECV II 型（79-1683）病毒（显示非细胞相关生长）反应的单克隆抗体。这些 MAb 还与 CCV 或 TGEV 发生反应。这些 MAb 可识别 peplomer (E2) 糖蛋白，并且在该 E2 蛋白中发现了许多抗原差异。这些结果表明，FIPV II 型和 FECV II 型病毒在抗原上更接近 TGEV 或 CCV，而不是 FIPV I 型病毒。此外，本研究中制备的单克隆抗体能够区分FIPV毒株79-1146和FECV毒株79-1683，这在以前的血清学方法中被认为是不可能的。
We prepared 31 monoclonal antibodies (MAbs) against either FIPV strain 79-1146 or FECV strain 79-1683, and tested them for reactivity with various coronaviruses by indirect flourescent antibody assay (IFA). Sixteen MAbs which reacted with all of the 11 strains of feline coronaviruses, also reacted with canine coronavirus (CCV) and transmissible gastroenteritis virus (TGEV). In many of them, the polypeptide specifity was the recognition of transmembrane (E1) protein of the virus. We succeeded in obtaining MAbs which did not react with eight strains FIPV Type I viruses (showing cell-associated growth) but reacted with FIPV Type II (79-1146, KU-1) and/or FECV Type II (79-1683) viruses (showing non-cell associated growth). These MAbs also reacted with CCV or TGEV. These MAbs recognized peplomer (E2) glycoprotein, and many antigenic differences were found in this E2 protein. These results suggest that FIPV Type II and FECV Type II viruses are antigenically closer to TGEV or CCV than to FIPV Type I viruses. Furthermore, the MAb prepared in this study has enabled discrimination between FIPV strain 79-1146 and FECV strain 79-1683, which was thought to be impossible by the previous serological method.
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DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J
影响因子：
9.4
作者：
FISCUS, SA;TERAMOTO, YA;PEDERSEN, NC
通讯作者：
PEDERSEN, NC
影响因子：
5.4
作者：
FISCUS, SA;TERAMOTO, YA
通讯作者：
TERAMOTO, YA
影响因子：
3.3
作者：
HOHDATSU, T;EIGUCHI, Y;MATUMOTO, M
通讯作者：
MATUMOTO, M
影响因子：
2.7
作者：
PEDERSEN, NC;WARD, J;MENGELING, WL
通讯作者：
MENGELING, WL