Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels.
Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels.
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DOI：
10.1016/j.biomaterials.2018.09.027
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发表时间：
2018-12
期刊：
影响因子：
14
通讯作者：
Cosgriff-Hernandez E
中科院分区：
文献类型：
--
作者：
Whitely M;Cereceres S;Dhavalikar P;Salhadar K;Wilems T;Smith B;Mikos A;Cosgriff-Hernandez E
基于聚合型高内相乳液(PolyHIPE)的组织工程支架的设计已成为一种很有前途的骨移植策略。我们之前报告了3D打印乳胶墨水的能力，以更好地模拟天然骨骼的结构和机械性能，同时精确匹配缺陷几何形状。在本研究中，利用可生物降解的大分子聚乙二醇二硫代苏糖醇，研究了氧化还原引发的水凝胶载体用于原位输送人骨髓间充质干细胞。调节水凝胶载体的性质，包括网络形成时间、溶胶凝胶率和溶胀度，以实现在没有外部刺激的情况下快速固化，目标细胞释放期为5-7天。这些原位载体使hMSCs在3D打印的聚HIPE移植物中的分布比标准悬浮种植有所改善。此外，载体负载的多聚HIPE支持hMSCs释放后持续的细胞活性和成骨分化。综上所述，这些发现证明了这种原位固化水凝胶载体在增强hMSCs在骨移植中的细胞分布和保留方面的潜力。虽然最初专注于促进骨再生，但将细胞包裹在水凝胶载体中而不依赖于可在大型移植物或组织中衰减的外部刺激的能力有望在组织工程中有广泛的应用。
The design of tissue engineered scaffolds based on polymerized high internal phase emulsions (polyHIPEs) has emerged as a promising bone grafting strategy. We previously reported the ability to 3D print emulsion inks to better mimic the structure and mechanical properties of native bone while precisely matching defect geometry. In the current study, redox-initiated hydrogel carriers were investigated for in situ delivery of human mesenchymal stem cells (hMSCs) utilizing the biodegradable macromer, poly(ethylene glycol)-dithiothreitol. Hydrogel carrier properties including network formation time, sol-gel fraction, and swelling ratio were modulated to achieve rapid cure without external stimuli and a target cell-release period of 5–7 days. These in situ carriers enabled improved distribution of hMSCs in 3D printed polyHIPE grafts over standard suspension seeding. Additionally, carrier-loaded polyHIPEs supported sustained cell viability and osteogenic differentiation of hMSCs post-release. In summary, these findings demonstrate the potential of this in situ curing hydrogel carrier to enhance the cell distribution and retention of hMSCs in bone grafts. Although initially focused on improving bone regeneration, the ability to encapsulate cells in a hydrogel carrier without relying on external stimuli that can be attenuated in large grafts or tissues is expected to have a wide range of applications in tissue engineering.
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