Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells.
Novel Technique for Retinal Nerve Cell Regeneration with Electrophysiological Functions Using Human Iris-Derived iPS Cells.
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DOI：
10.3390/cells10040743
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发表时间：
2021-03-28
期刊：
影响因子：
6
通讯作者：
Sasaki H
中科院分区：
文献类型：
--
作者：
Yamamoto N;Hiramatsu N;Ohkuma M;Hatsusaka N;Takeda S;Nagai N;Miyachi EI;Kondo M;Imaizumi K;Horiguchi M;Kubo E;Sasaki H
已经描述了使用诱导多能干细胞（iPS）细胞的眼科再生医学，但这些研究使用了来自成纤维细胞的iPS细胞。在这里，我们产生了来自虹膜细胞的iPS细胞，虹膜细胞与视网膜一样从视杯的内层发育，以再生视网膜神经。我们首先在人虹膜组织中鉴定出p75 NTR阳性细胞，p75 NTR是视网膜组织干细胞和祖细胞的标志物。然后，我们重新编程培养的p75 NTR阳性虹膜组织干/祖细胞（H-iris干/祖细胞），以创造虹膜衍生的iPS（H-iris iPS）细胞的第一次。这些细胞对iPS细胞标志物呈阳性，并显示出分化成三个胚层的多能性。当H-iris iPS细胞预分化为神经干/祖细胞时，并非所有细胞都对神经干/祖细胞和神经细胞标志物呈阳性。当这些细胞预分化为神经干/祖细胞，用p75 NTR分选，并用作分化为视网膜神经细胞的培养基时，细胞分化为具有电生理功能的恢复蛋白阳性细胞。在不同的培养基中，H-iris iPS细胞分化为具有电生理功能的视网膜神经节细胞标志物阳性细胞。这是首次证明H-虹膜iPS细胞分化为视网膜神经元，在生理上发挥神经元的功能。
Regenerative medicine in ophthalmology that uses induced pluripotent stem cells (iPS) cells has been described, but those studies used iPS cells derived from fibroblasts. Here, we generated iPS cells derived from iris cells that develop from the same inner layer of the optic cup as the retina, to regenerate retinal nerves. We first identified cells positive for p75NTR, a marker of retinal tissue stem and progenitor cells, in human iris tissue. We then reprogrammed the cultured p75NTR-positive iris tissue stem/progenitor (H-iris stem/progenitor) cells to create iris-derived iPS (H-iris iPS) cells for the first time. These cells were positive for iPS cell markers and showed pluripotency to differentiate into three germ layers. When H-iris iPS cells were pre-differentiated into neural stem/progenitor cells, not all cells became positive for neural stem/progenitor and nerve cell markers. When these cells were pre-differentiated into neural stem/progenitor cells, sorted with p75NTR, and used as a medium for differentiating into retinal nerve cells, the cells differentiated into Recoverin-positive cells with electrophysiological functions. In a different medium, H-iris iPS cells differentiated into retinal ganglion cell marker-positive cells with electrophysiological functions. This is the first demonstration of H-iris iPS cells differentiating into retinal neurons that function physiologically as neurons.
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影响因子：
25
作者：
Fischer, AJ;Reh, TA
通讯作者：
Reh, TA
影响因子：
1.7
作者：
Bianconi, Eva;Piovesan, Allison;Canaider, Silvia
通讯作者：
Canaider, Silvia
DOI：
10.1073/pnas.71.12.5052
发表时间：
1974-01-01
影响因子：
11.1
作者：
EGUCHI, G;ABE, SI;WATANABE, K
通讯作者：
WATANABE, K
影响因子：
16.2
作者：
Kawai, F;Horiguchi, M;Miyachi, E
通讯作者：
Miyachi, E
影响因子：
5.9
作者：
Kamao, Hiroyuki;Mandai, Michiko;Okamoto, Satoshi;Sakai, Noriko;Suga, Akiko;Sugita, Sunao;Kiryu, Junichi;Takahashi, Masayo
通讯作者：
Takahashi, Masayo