Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.
Intestinal microbiota in healthy U.S. young children and adults--a high throughput microarray analysis.
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DOI：
10.1371/journal.pone.0064315
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Satokari R
中科院分区：
文献类型：
--
作者：
Ringel-Kulka T;Cheng J;Ringel Y;Salojärvi J;Carroll I;Palva A;de Vos WM;Satokari R
一般认为，婴儿的微生物群是在生命的最初1-2年建立起来的。然而，关于其特征及其连续多年与成人样微生物群的比较的数据很少。采用高通量细菌系统发育微阵列分析，对美国北卡罗来纳地区健康幼儿（1-4岁）和健康成人的肠道微生物群进行表征和比较。采用针对16S rRNA V1和V6区域的人类肠道芯片（HITChip）系统发育芯片和定量PCR技术，对1-4岁健康儿童（n = 28）和21-60岁健康成人（n = 23）的肠道微生物群进行了详细的表征和比较。HITChip芯片数据显示，放线菌、芽胞杆菌、梭菌簇IV和拟杆菌门是主要的类门类群，在幼儿和成人之间表现出差异。类门群Clostridium cluster XIVa在幼儿和成人中同样占优势，因此被认为是在早期建立的。在类属水平上，26个属的丰度在幼儿和成人之间存在3.6倍（高或低）的显著差异。美国儿童的双歧杆菌种类丰度比同一地区的成年人高3.5倍。然而，幼儿的微生物群的多样性不如成人。我们发现，成人样肠道微生物群的建立比以前报道的要晚。描述微生物群及其在生命早期的发育可能有助于确定干预策略的“机会之窗”，从而促进健康，预防或减轻疾病进程。
It is generally believed that the infant's microbiota is established during the first 1–2 years of life. However, there is scarce data on its characterization and its comparison to the adult-like microbiota in consecutive years. To characterize and compare the intestinal microbiota in healthy young children (1–4 years) and healthy adults from the North Carolina region in the U.S. using high-throughput bacterial phylogenetic microarray analysis. Detailed characterization and comparison of the intestinal microbiota of healthy children aged 1–4 years old (n = 28) and healthy adults of 21–60 years (n = 23) was carried out using the Human Intestinal Tract Chip (HITChip) phylogenetic microarray targeting the V1 and V6 regions of 16S rRNA and quantitative PCR. The HITChip microarray data indicate that Actinobacteria, Bacilli, Clostridium cluster IV and Bacteroidetes are the predominant phylum-like groups that exhibit differences between young children and adults. The phylum-like group Clostridium cluster XIVa was equally predominant in young children and adults and is thus considered to be established at an early age. The genus-like level show significant 3.6 fold (higher or lower) differences in the abundance of 26 genera between young children and adults. Young U.S. children have a significantly 3.5-fold higher abundance of Bifidobacterium species than the adults from the same location. However, the microbiota of young children is less diverse than that of adults. We show that the establishment of an adult-like intestinal microbiota occurs at a later age than previously reported. Characterizing the microbiota and its development in the early years of life may help identify ‘windows of opportunity’ for interventional strategies that may promote health and prevent or mitigate disease processes.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.9
作者：
Frank, Daniel N.;Robertson, Charles E.;Hamm, Christina M.;Kpadeh, Zegbeh;Zhang, Tianyi;Chen, Hongyan;Zhu, Wei;Sartor, R. Balfour;Boedeker, Edgar C.;Harpaz, Noam;Pace, Norman R.;Li, Ellen
通讯作者：
Li, Ellen
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
3.5
作者：
Gerritsen, Jacoline;Smidt, Hauke;Rijkers, Ger T.;de Vos, Willem M.
通讯作者：
de Vos, Willem M.