The effects of confinement on neuronal growth cone morphology and velocity.
The effects of confinement on neuronal growth cone morphology and velocity.
复制标题
限制对神经元生长锥形态和速度的影响。
DOI：
10.1016/j.biomaterials.2014.04.097
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Urbach, Jeffrey S.
中科院分区：
文献类型：
--
作者：
Smirnov, Michael S.;Cabral, Katelyn A.;Geller, Herbert M.;Urbach, Jeffrey S.
通过使用图案化基底优化生长锥引导对于设计再生基底以帮助从神经元损伤中恢复是重要的。利用激光烧蚀，我们设计了微米尺度的图案，能够将分离的小鼠小脑颗粒神经元生长锥限制在1.5至12 μm的不同宽度的通道中。在这些通道中的生长锥动态观察使用时间推移显微镜。与12 μm和未图案化的基底相比，在1.5和6 μm之间的通道中生长锥面积减小。生长锥纵横比也受到影响，因为较窄的通道迫使生长锥变成狭窄的细长形状。与在未图案化的基底上生长相比，在1.5和12 μm之间的均匀通道中生长锥前进的总体速率没有差异。生长锥前进、暂停和缩回的时间百分比也相似。然而，生长锥确实对限制的变化做出了反应：狭窄通道中的生长锥在遇到宽区域时迅速加速，然后在进入另一个狭窄区域时减速。我们的研究结果表明，神经突延伸的速度是不受限制的程度，但不响应限制的变化。
Optimizing growth cone guidance through the use of patterned substrates is important for designing regenerative substrates to aid in recovery from neuronal injury. Using laser ablation, we designed micron-scale patterns capable of confining dissociated mouse cerebellar granule neuron growth cones to channels of different widths ranging from 1.5 to 12 μm. Growth cone dynamics in these channels were observed using time lapse microscopy. Growth cone area was decreased in channels between 1.5 and 6 μm as compared to that in 12 μm and unpatterned substrates. Growth cone aspect ratio was also affected as narrower channels forced growth cones into a narrow, elongated shape. There was no difference in the overall rate of growth cone advance in uniform channels between 1.5 and 12 μm as compared to growth on unpatterned substrates. The percentage of time growth cones advanced, paused, and retracted was also similar. However, growth cones did respond to changes in confinement: growth cones in narrow lanes rapidly sped up when encountering a wide region and then slowed down as they entered another narrow region. Our results suggest that the rate of neurite extension is not affected by the degree of confinement, but does respond to changes in confinement.
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