Identification of peroxiredoxin-5 in bovine cauda epididymal sperm.
Identification of peroxiredoxin-5 in bovine cauda epididymal sperm.
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DOI：
10.1007/s11010-013-1876-3
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发表时间：
2014-02
影响因子：
4.3
通讯作者：
Raychoudhury, Samir
中科院分区：
文献类型：
--
作者：
Nagdas, Subir K.;Buchanan, Teresa;Raychoudhury, Samir
发育中的精子需要在附睾腔环境中进行一系列睾丸后修饰以实现成熟;这涉及几种表面修饰，包括质膜脂质、蛋白质、碳水化合物的变化和顶体外膜的改变。因此，附睾成熟可以使精子获得向前运动和受精的能力。本研究的目的是鉴定成熟依赖蛋白，并研究它们在功能活性精子产生中的作用。精子头部和尾部质膜组分的凝集素印迹分析确定了一个17.5kDa的小麦胚芽凝集素（WGA）结合多肽存在于精子尾部质膜，而不是在精子头部质膜。在几条WGA染色条带中，仅在附睾尾液中检测到17.5kDa WGA结合多肽条带，而在附睾头液中未检测到，这表明17.5kDa WGA结合多肽从附睾尾分泌，并在附睾运输期间与尾部精子质膜结合。对17.5kDa多肽的蛋白质组学鉴定产生了13个与过氧化物氧还蛋白-5（PRDX 5）蛋白（牛）序列相匹配的肽。我们建议，牛尾精子PRDX 5作为一种抗氧化酶在附睾环境中，这是至关重要的保护活精子人口对内源性或外源性过氧化物造成的损害。
Developing spermatozoa require a series of post-testicular modifications within the luminal environment of the epididymis to achieve maturation; this involves several surface modifications including changes in plasma membrane lipids, proteins, carbohydrates, and alterations in the outer acrosomal membrane. Epididymal maturation can therefore allow sperm to gain forward motility and fertilization capabilities. The objective of this study was to identify maturation dependent protein(s) and to investigate their role with the production of functionally competent spermatozoa. Lectin blot analyses of caput and cauda sperm plasma membrane fractions identified a 17.5kDa Wheat Germ Agglutinin (WGA) binding polypeptide present in the cauda sperm plasma membrane not in the caput sperm plasma membrane. Among the several WGA stained bands, the presence of a 17.5kDa WGA binding polypeptide band was detected only in cauda epididymal fluid not in caput epididymal fluid suggesting that the 17.5kDa WGA-binding polypeptide is secreted from the cauda epididymis and binds to the cauda sperm plasma membrane during epididymal transit. Proteomic identification of the 17.5kDa polypeptide yielded 13 peptides that matched the sequence of peroxiredoxin-5 (PRDX5) protein (Bos Taurus). We propose that bovine cauda sperm PRDX5 acts as an antioxidant enzyme in the epididymal environment, which is crucial in protecting the viable sperm population against the damage caused by endogeneous or exogeneous peroxide.
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影响因子：
3.6
作者：
DACHEUX, JL;DACHEUX, F;PAQUIGNON, M
通讯作者：
PAQUIGNON, M
影响因子：
4.1
作者：
Dacheux, Jean-Louis;Belleannee, Clemence;Dacheux, Francoise
通讯作者：
Dacheux, Francoise
影响因子：
3.6
作者：
Belleannee, Clemence;Calvo, Ezequiel;Sullivan, Robert
通讯作者：
Sullivan, Robert
DOI：
10.3109/01485018108987531
发表时间：
1981-01-01
期刊：
ARCHIVES OF ANDROLOGY
影响因子：
--
作者：
KOEHLER, JK
通讯作者：
KOEHLER, JK
影响因子：
2.9
作者：
Cooper, Trevor G.
通讯作者：
Cooper, Trevor G.