The Human Mesenteric Lymph Node Microbiome Differentiates Between Crohn's Disease and Ulcerative Colitis.
The Human Mesenteric Lymph Node Microbiome Differentiates Between Crohn's Disease and Ulcerative Colitis.
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DOI：
10.1093/ecco-jcc/jjy136
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Dunne CP
中科院分区：
文献类型：
--
作者：
Kiernan MG;Coffey JC;McDermott K;Cotter PD;Cabrera-Rubio R;Kiely PA;Dunne CP
肠系膜淋巴结是易位细菌激发和发展免疫反应的场所。因此，了解炎症性肠病中肠系膜淋巴结的微生物组很重要。仅在一项先前的研究中使用非培养方法分析了克罗恩病肠系膜淋巴结的细菌谱。本研究旨在研究克罗恩病和溃疡性结肠炎患者的肠系膜淋巴结微生物群。从接受切除术的克罗恩病和溃疡性结肠炎患者收集肠系膜淋巴结。从肠系膜淋巴结中提取总DNA，并评估是否存在细菌DNA [16S]。所有工作均在无菌环境中使用无菌技术完成。对16S DNA阳性的样品进行下一代测序，并确定细菌门和种的身份。克罗恩病肠系膜淋巴结的微生物特征与溃疡性结肠炎中观察到的微生物特征明显不同。厚壁菌门的相对丰度在溃疡性结肠炎患者的淋巴结中更高，而变形菌门在克罗恩病中更丰富。尽管克罗恩病患者肠系膜淋巴结的物种多样性降低，但这些淋巴结中含有更多数量的不占优势的门，主要是梭菌。本研究证实克罗恩病和溃疡性结肠炎肠系膜淋巴结微生物组之间存在明显差异。这种微生物差异可以帮助诊断克罗恩病或溃疡性结肠炎，特别是在切除时不确定的结肠炎病例中，或帮助解释其发展和进展的机制。
Mesenteric lymph nodes are sites in which translocated bacteria incite and progress immunological responses. For this reason, understanding the microbiome of mesenteric lymph nodes in inflammatory bowel disease is important. The bacterial profile of Crohn’s disease mesenteric lymph nodes has been analysed using culture-independent methods in only one previous study. This study aimed to investigate the mesenteric lymph node microbiota from both Crohn’s disease and ulcerative colitis patients. Mesenteric lymph nodes were collected from Crohn’s disease and ulcerative colitis patients undergoing resection. Total DNA was extracted from mesenteric lymph nodes and assessed for the presence of bacterial DNA [16S]. All work was completed in a sterile environment using aseptic techniques. Samples positive for 16S DNA underwent next-generation sequencing, and the identity of bacterial phyla and species were determined. Crohn’s disease mesenteric lymph nodes had a distinctly different microbial profile to that observed in ulcerative colitis. The relative abundance of Firmicutes was greater in nodes from ulcerative colitis patients, whereas Proteobacteria were more abundant in Crohn’s disease. Although species diversity was reduced in the mesenteric lymph nodes of patients with Crohn’s disease, these lymph nodes contained greater numbers of less dominant phyla, mainly Fusobacteria. This study confirms that there are distinct differences between the Crohn’s disease and ulcerative colitis mesenteric lymph node microbiomes. Such microbial differences could aid in the diagnosis of Crohn’s disease or ulcerative colitis, particularly in cases of indeterminate colitis at time of resection, or help explain their mechanisms of development and progression.
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影响因子：
6.4
作者：
Cronin O;Barton W;Skuse P;Penney NC;Garcia-Perez I;Murphy EF;Woods T;Nugent H;Fanning A;Melgar S;Falvey EC;Holmes E;Cotter PD;O'Sullivan O;Molloy MG;Shanahan F
通讯作者：
Shanahan F
影响因子：
4.3
作者：
Dong LN;Wang JP;Liu P;Yang YF;Feng J;Han Y
通讯作者：
Han Y
影响因子：
24.5
作者：
ARIES, V;CROWTHER, JS;WILLIAMS, RE
通讯作者：
WILLIAMS, RE
影响因子：
4.9
作者：
Forbes, Jessica D.;Van Domselaar, Gary;Bernstein, Charles N.
通讯作者：
Bernstein, Charles N.
DOI：
10.1073/pnas.0706625104
发表时间：
2007-08-21
影响因子：
11.1
作者：
Frank, Daniel N.;Amand, Allison L. St.;Pace, Norman R.
通讯作者：
Pace, Norman R.