Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.
Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare.
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DOI：
10.1038/s41598-023-31540-9
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发表时间：
2023-03-21
影响因子：
4.6
通讯作者：
Card C
中科院分区：
文献类型：
--
作者：
Diel de Amorim M;Dong L;Byron M;Foster RA;Klein C;Saleh M;Saleh T;Card C
催产素是一种激素，具有以下功能：生殖，母性结合，乳汁排出和喂养/社会行为，并且据报道存在于各种组织中。我们的目标是表征催产素和亮氨酰和胱氨酸氨肽酶（LNPEP/催产素酶），催产素在子宫内膜中的关键调节因子。我们用ELISA法测定了非妊娠组（n = 5）和妊娠组（n = 6）以及围产期和产后组（n = 18）从排卵（D 0）到D21-22的血清和组织LNPEP。使用蛋白质测定和LNPEP ELISA评价胎盘（n = 7）和间情期胎盘的匀浆组织（n = 6）。还通过蛋白质印迹、免疫组织化学和液相色谱-质谱（LC-MS/MS）鉴定组织中的LNPEP和OXT蛋白。同时对子宫内膜、子宫肌层、垂体和黄体进行LNPEP和OXT的原位杂交。血清LNPEP浓度相似。胎盘LNPEP U/mg蛋白在体部和孕角最高。各组织LNPEP U/mg蛋白的最高值和最低值依次为：子宫肌层>卵泡壁>子宫内膜>肾脏> CL >肝脏。催产素在马垂体、CL和胎盘中被确定，并且可能以自分泌或旁分泌方式起作用，而LNPEP可能全身和局部地起作用以调节OXT的可用性。
Oxytocin is a hormone with functions in: reproduction, maternal bonding, milk ejection, and feeding/social behavior, and is reported to be present in a variety of tissues. Our goal is to characterize oxytocin and leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase), a key regulator of oxytocin in mares. We measured serum and tissue LNPEP by ELISA from ovulation (D0) until D21–22 in non-pregnant (n = 5) and pregnant mares (n = 6); and in periparturient and postpartum mares (n = 18). Placenta (n = 7) and homogenized tissue of diestrus mares (n = 6) were evaluated using protein determinations and LNPEP ELISAs. Identification of LNPEP and OXT protein in tissues was also performed via western blot, immunohistochemistry and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). Serum LNPEP concentration were similar. Placental LNPEP U/mg protein was highest in the body and pregnant horn. The highest to lowest LNPEP U/mg protein by tissue were: myometrium > follicle wall > endometrium > kidney > CL > liver. Oxytocin was identified in the equine pituitary, CL and placenta and is likely to act in autocrine or paracrine manner, while LNPEP may act systemically and locally to regulate the availability of OXT.
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影响因子：
3.2
作者：
Kobayashi, H;Nomura, S;Mizutani, S
通讯作者：
Mizutani, S
DOI：
10.1007/bf01972292
发表时间：
1982-01-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
MIZUTANI, S;OKANO, K;YAMADA, M
通讯作者：
YAMADA, M
影响因子：
4.1
作者：
Chatterjee, Oishi;Patil, Krutika;Prasad, T. S. Keshava
通讯作者：
Prasad, T. S. Keshava
马基因组的蛋白质编码区域的注释。
影响因子：
3.7
作者：
Hestand MS;Kalbfleisch TS;Coleman SJ;Zeng Z;Liu J;Orlando L;MacLeod JN
通讯作者：
MacLeod JN
影响因子：
2.8
作者：
Bae, SE;Watson, ED
通讯作者：
Watson, ED