The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer.
The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer.
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DOI：
10.1186/1757-2215-7-76
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发表时间：
2014-07-27
影响因子：
4
通讯作者：
Ohmichi M
中科院分区：
文献类型：
--
作者：
Takai M;Terai Y;Kawaguchi H;Ashihara K;Fujiwara S;Tanaka T;Tsunetoh S;Tanaka Y;Sasaki H;Kanemura M;Tanabe A;Ohmichi M
上皮间质转化（EMT）是肿瘤侵袭和转移的重要环节。这一过程的一个关键分子特征是下调E-钙粘蛋白的表达，这主要是由蜗牛相关的锌指转录因子控制。本研究的目的是评估EMT相关蛋白（E-cadherin和Snail）表达对卵巢癌患者预后的影响。应用免疫组化方法对174例卵巢癌原发灶和34例卵巢癌转移灶的组织芯片标本进行分析，探讨其蛋白表达与临床病理特征及预后的关系。在36.8%的原发性肿瘤和30.4%、35.7%、37.7%和52.7%的I、II、III和IV期肿瘤中分别观察到E-钙粘蛋白表达降低。Snail在33.9%的原发肿瘤中表达阳性。EMT阳性状态的发生率，即E-cadherin表达减少和Snail核表达减少，在有腹膜播散的患者中显著高于无腹膜播散的患者（p < 0.05）。EMT状态与无进展生存期和总生存期显著相关（p <0.01）。多因素分析显示EMT阳性状态是无进展生存期（P < 0.05）和总生存期（P < 0.01）的显著预测因子。这些数据表明，EMT状态与卵巢癌患者的腹膜转移以及无进展生存期和总生存期显著相关。因此，阐明和控制EMT信号是卵巢癌分子靶向治疗的一个有前途的途径。
The epithelial-mesenchymal-transition (EMT) is an important step in the invasion and metastasis of cancer. A critical molecular feature of this process is the downregulation of the E-cadherin expression, which is primarily controlled by Snail-related zinc-finger transcription factors. The aim of this study was to evaluate the prognostic impact of the expression of EMT-related proteins (E-cadherin and Snail) in patients with ovarian cancer. An immunohistochemical analysis was conducted using tissue microarray samples of 174 primary tumors and 34 metastases of ovarian carcinoma, and the relationships between the protein expression, clinicopathological features and outcomes were investigated. A reduced E-cadherin expression was observed in 36.8% of the primary tumors and 30.4%, 35.7%, 37.7% and 52.7% of the stage I, II, III and IV tumors, respectively. The nuclear expression of Snail was positive in 33.9% of the primary tumors. The rate of an EMT-positive status, as represented by both a reduced E-cadherin expression and a nuclear expression of Snail, was significantly higher in the patients with peritoneal dissemination than in those without (p < 0.05). The EMT status was significantly associated with both the progression-free survival and overall survival (p <0.01). A multivariate analysis showed an EMT-positive status to be a significant predictor of both the progression-free survival (p < 0.05) and overall survival (P < 0.01). These data indicate that the EMT status is significantly associated with peritoneal metastasis and both the progression-free survival and overall survival in patients with ovarian cancer. Therefore, clarifying and controlling EMT signaling is a promising approach to molecular targeted therapy for ovarian cancer.
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影响因子：
3.6
作者：
Tanaka Y;Terai Y;Kawaguchi H;Fujiwara S;Yoo S;Tsunetoh S;Takai M;Kanemura M;Tanabe A;Ohmichi M
通讯作者：
Ohmichi M
影响因子：
4.7
作者：
SAKURAGI, N;NISHIYA, M;FUJIMOTO, S
通讯作者：
FUJIMOTO, S
影响因子：
8
作者：
Grooteclaes, ML;Frisch, SM
通讯作者：
Frisch, SM
影响因子：
21.3
作者：
Batlle, E;Sancho, E;de Herreros, AG
通讯作者：
de Herreros, AG
影响因子：
7.5
作者：
Takeichi, Masatoshi
通讯作者：
Takeichi, Masatoshi