High throughput microencapsulation of Bacillus subtilis in semi-permeable biodegradable polymersomes for selenium remediation.
High throughput microencapsulation of Bacillus subtilis in semi-permeable biodegradable polymersomes for selenium remediation.
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DOI：
10.1007/s00253-016-7896-7
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发表时间：
2017-01
影响因子：
5
通讯作者：
van de Ven, Anne L.
中科院分区：
文献类型：
--
作者：
Barlow, Jacob;Gozzi, Kevin;Kelley, Chase P.;Geilich, Benjamin M.;Webster, Thomas J.;Chai, Yunrong;Sridhar, Srinivas;van de Ven, Anne L.
将细菌封装在受限的微环境中可以促进特殊行为的表现。使用双乳液液滴生成微流体合成，将活枯草芽孢杆菌封装在由聚（乙二醇）-b-聚（D,L-乳酸）组成的半透膜中。这种 mPEG-PDLLA 膜具有足够的渗透性，允许细菌指数生长、代谢物诱导的基因表达和快速生物膜生长。可生物降解的微粒在几天内保持结构完整性，并且可以随着时间或持续的细菌活性成功降解。微囊枯草芽孢杆菌成功捕获并含有添加到聚合物囊泡外部的亚硒酸钠，将亚硒酸盐转化为元素硒纳米颗粒，选择性地保留在聚合物膜内。这种使用聚合物囊泡对硒的修复具有降低环境硒污染的毒性的巨大潜力，并且允许从不适合常规废物或水处理的区域收获硒。
Encapsulating bacteria within constrained microenvironments can promote the manifestation of specialized behaviors. Using double-emulsion droplet-generating microfluidic synthesis, live Bacillus subtilis were encapsulated in a semi-permeable membrane comprised of poly(ethylene glycol)-b-poly(D,L-lactic acid). This mPEG-PDLLA membrane was sufficiently permeable to permit exponential bacterial growth, metabolite-induced gene expression, and rapid biofilm growth. The biodegradable microparticles retained structural integrity for several days, and could be successfully degraded with time or sustained bacterial activity. Microencapsulated B. subtilis successfully captured and contained sodium selenite added outside the polymersomes, converting the selenite into elemental selenium nanoparticles that were selectively retained inside the polymer membrane. This remediation of selenium using polymersomes has high potential for reducing the toxicity of environmental selenium contamination, as well as allowing selenium to be harvested from areas not amenable to conventional waste or water treatment.
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影响因子：
4.6
作者：
Chen, Si;Cheng, Si-Xue;Zhuo, Ren-Xi
通讯作者：
Zhuo, Ren-Xi
影响因子：
5
作者：
Makadia HK;Siegel SJ
通讯作者：
Siegel SJ
影响因子：
--
作者：
Prakash S;Tomaro-Duchesneau C;Saha S;Cantor A
通讯作者：
Cantor A
影响因子：
10.5
作者：
Lopez, Daniel;Vlamakis, Hera;Kolter, Roberto
通讯作者：
Kolter, Roberto
DOI：
10.3390/molecules200813894
发表时间：
2015-07-31
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Estevam EC;Witek K;Faulstich L;Nasim MJ;Latacz G;Domínguez-Álvarez E;Kieć-Kononowicz K;Demasi M;Handzlik J;Jacob C
通讯作者：
Jacob C