Identification of novel genetic regions associated with resistance to European canker in apple
Identification of novel genetic regions associated with resistance to European canker in apple
复制标题
鉴定与苹果抗欧洲溃疡病相关的新遗传区域
DOI：
10.1101/2021.12.20.473552
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Karlström A
中科院分区：
文献类型：
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作者：
Karlström A
背景欧洲溃疡病是由真菌病原体Neonectria ditissima引起的，是世界上苹果产区的一种经济破坏性疾病-特别是在温度适中和降雨量高的地区。该病原菌具有广泛的寄主范围的硬木多年生物种，造成树干溃疡，枯死和分支病变，在其主机。虽然苹果接穗种质进行部分抗性的疾病已经描述，仍然知之甚少的遗传基础，这种定量resistance.ResultsResistance to Neonectria ditissima进行了研究，在一个多亲群体的苹果接穗使用几种表型方法。所研究的群体由来自多个家庭的个体组成，这些家庭通过一个共同的系谱联系在一起。在三个实验中，每个人在人口中的疾病程度进行了评估：人工接种的分离休眠芽，盆栽树在温室中，并在一个复制的现场实验。使用基于家系的分析（PBA）研究疾病差异的遗传基础。三个数量性状位点（QTL），连锁群（LG）6，8和10被确定在一个以上的表型策略。另外4个QTL，位于LG 2、5、15和16上，仅在田间试验中被鉴定。在双亲群体中进一步验证了LG 2和16上的QTL。QTL效应大小为小到中等，单个QTL解释的方差为4.3%至19%。随后的QTL单倍型的分析揭示了这种疾病的动态响应，在该单倍型的估计效果不同的字段time-points.ConclusionsThis研究描述了第一个确定的QTL与耐N。典型苹果接穗种质本研究结果表明，苹果育种中常用的种质资源中存在的QTL对苹果的抗氮性具有低至中等的效应。ditissima。因此，需要考虑多个QTL以通过育种提高抗性。
BackgroundEuropean canker, caused by the fungal pathogenNeonectria ditissima,is an economically damaging disease in apple producing regions of the world – especially in areas with moderate temperatures and high rainfall. The pathogen has a wide host range of hardwood perennial species, causing trunk cankers, dieback and branch lesions in its hosts. Although apple scion germplasm carrying partial resistance to the disease has been described, little is still known of the genetic basis for this quantitative resistance.ResultsResistance toNeonectria ditissimawas studied in a multiparental population of apple scions using several phenotyping methods. The studied population consists of individuals from multiple families connected through a common pedigree. The degree of disease of each individual in the population was assessed in three experiments: artificial inoculations of detached dormant shoots, potted trees in a glasshouse and in a replicated field experiment. The genetic basis of the differences in disease was studied using a pedigree-based analysis (PBA). Three quantitative trait loci (QTL), on linkage groups (LG) 6, 8 and 10 were identified in more than one of the phenotyping strategies. An additional four QTL, on LG 2, 5, 15 and 16 were only identified in the field experiment. The QTL on LG2 and 16 were further validated in a biparental population. QTL effect sizes were small to moderate with 4.3 to 19% of variance explained by a single QTL. A subsequent analysis of QTL haplotypes revealed a dynamic response to this disease, in which the estimated effect of a haplotype varied over the field time-points.ConclusionsThis study describes the first identified QTL associated with resistance toN. ditissimain apple scion germplasm. The results from this study show that QTL present in germplasm commonly used in apple breeding have a low to medium effect on resistance toN. ditissima. Hence, multiple QTL will need to be considered to improve resistance through breeding.
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影响因子：
3.2
作者：
Beresford, Robert M.;Kim, Kwang Soo
通讯作者：
Kim, Kwang Soo
影响因子：
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作者：
Amponsah, N. T.;Scheper, R. W. A.;Jesson, L. K.
通讯作者：
Jesson, L. K.
防护林带是苹果树新克霉感染的潜在接种源吗
影响因子：
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作者：
M.Walter;M. Glaister;N. R. Clarke;H. Lutz;Z. Eld;N. T. Amponsah;N. Shaw
通讯作者：
N. Shaw
在对苹果对新克霉的抗性进行表型分析时，离体枝条处理不能替代整树测定
影响因子：
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作者：
R. Scheper;B. M. Fisher;T. Taylor;D. Hedderley
通讯作者：
D. Hedderley
苹果树伤口被Neonectria ditissima感染需要多少分生孢子
影响因子：
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作者：
M. Walter;S. Roy;B. M. Fisher;L. Mackle;N. T. Amponsah;T. Curnow;R. Campbell;P. Braun;A. Reineke;R. Scheper
通讯作者：
R. Scheper