Conductive Conjugated Polymer Nanocapacitors for Localized Electrical Neurostimulation
Conductive Conjugated Polymer Nanocapacitors for Localized Electrical Neurostimulation
复制标题
用于局部电神经刺激的导电共轭聚合物纳米电容器
DOI：
10.1021/acsanm.2c03152
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发表时间：
2022
影响因子：
5.9
通讯作者：
Romero, Gabriela
中科院分区：
文献类型：
--
作者：
Martinez-Cartagena, Manuel E.;Muzzio, Nicolas E.;Guntnur, Rohini Thevi;Fisher, Vanessa;Hebbale, Skanda;Rodgers, Tina;Romero-Garcia, Jorge;Romero, Gabriela
细胞信号的无创操作在基础神经科学研究和开发神经疾病治疗、组织工程和再生医学方法中至关重要。在这项工作中，仿生合成导电共聚物3,4-乙烯二氧噻吩(EDOT)-吡咯纳米粒子（RB02 NPs）用于无线和局部刺激神经元。采用1H核磁共振对聚合过程进行监测。利用拉曼光谱、傅里叶红外光谱和动态光散射对RB02 NPs进行了表征。采用恒流充放电法、伏安法和电化学阻抗法对其电化学性能进行了表征。对于神经元的电刺激，RB02 NPs在使用铂电极的NP悬浮液中施加1 V充电。流式细胞术检测NPs对ND7/23神经元杂交细胞系活性的影响。通过细胞骨架染色和树突数、长度等形态学参数定量评价ND7/23的分化情况。通过Fluo-4的动态荧光变化检测钙离子内流，研究初级皮层神经元的刺激。RB02 NPs对ND7/23细胞无毒性作用。此外，带电荷的NPs在添加后短时间内（<6 h）促进细胞分化。充电的RB02 NPs大大增加了皮层神经元的活动。总之，生物相容性共聚物edot -吡咯纳米颗粒在神经活动的远程控制方面具有很大的潜力。
Noninvasive manipulation of cell signaling is critical in basic neuroscience research and in developing therapies for neurological disorders and tissue engineering and regenerative medicine approaches. In this work, biomimetic synthesized conductive copolymer 3,4-ethylenedioxythiophene (EDOT)-Pyrrole nanoparticles (RB02 NPs) were used for wireless and localized stimulation of neurons.1H nuclear magnetic resonance was used to monitor the polymerization. RB02 NPs were characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, and dynamic light scattering. The electrochemical properties were characterized by galvanostatic charge–discharge, voltammetry, and electrochemical impedance spectroscopy. For electrical stimulation of neurons, RB02 NPs were charged by applying 1 V to a NP suspension using platinum electrodes. The effect of NPs on ND7/23 neuron hybrid cell line viability was assessed by live/dead staining using flow cytometry. ND7/23 differentiation was evaluated by cell cytoskeleton staining and quantification of morphological parameters such as the dendrite number and length. Primary cortex neuron stimulation was studied by calcium ion influx detectable through the dynamic fluorescence changes of Fluo-4. RB02 NPs presented no toxicity toward ND7/23 cells. Furthermore, charged NPs enhanced cell differentiation at short times after addition (<6 h). Charged RB02 NPs largely increased the cortex neuronal activity. Altogether, biocompatible copolymer EDOT-Pyrrole nanoparticles present great potential for remote control of neural activities.
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影响因子：
4.3
作者：
Colombo E;Feyen P;Antognazza MR;Lanzani G;Benfenati F
通讯作者：
Benfenati F
DOI：
10.1002/adma.202103208
发表时间：
2021-12
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Yang X;McGlynn E;Das R;Paşca SP;Cui B;Heidari H
通讯作者：
Heidari H
影响因子：
7.4
作者：
Carton, Ixaskun;Brisson, Alain R.;Richter, Ralf P.
通讯作者：
Richter, Ralf P.
影响因子：
3.6
作者：
Nichols EL;Green LA;Smith CJ
通讯作者：
Smith CJ
影响因子：
13.6
作者：
Kozielski KL;Jahanshahi A;Gilbert HB;Yu Y;Erin Ö;Francisco D;Alosaimi F;Temel Y;Sitti M
通讯作者：
Sitti M