Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging.
Rapid mapping of visual receptive fields by filtered back projection: application to multi-neuronal electrophysiology and imaging.
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DOI：
10.1113/jphysiol.2014.276642
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发表时间：
2014-11-15
期刊：
影响因子：
--
通讯作者：
Lagnado L
中科院分区：
文献类型：
--
作者：
Johnston J;Ding H;Seibel SH;Esposti F;Lagnado L
视觉系统中的神经元在其感受野（RF）的时空特性方面差异很大，理解这些变化是阐明视觉信息如何处理的关键。我们提出了一种新的方法来映射RF的基础上过滤反投影（FBP），用于断层重建的算法。为了估计RF，在视网膜上以伪随机位置和至少五个方向闪烁一系列条。我们将这种方法应用于视网膜神经元，并表明它可以准确地恢复记录在多电极阵列上的神经节细胞的空间RF和脉冲响应。我们还证明了它的效用，在体内成像映射的RF阵列的双极细胞突触表达基因编码的Ca 2+指标。我们发现，FBP提供了几个优点，比常用的尖峰触发平均（STA）：（i）ON和OFF成分的RF可以分离;（ii）脉冲响应可以重建在采样率为125 Hz，而不是刷新率的监视器;（iii）FBP揭示了使用STA不明显的神经元的响应特性，包括显示取向选择性的那些，或发射在低的平均尖峰率;和（iv）的FBP方法是快速的，允许在4分钟内重建的视野中的所有双极细胞突触终端的RF。使用的FBP将有利于调查的视觉系统，采用电生理学或光学报告，以衡量跨群体的神经元的活动。
Neurons in the visual system vary widely in the spatiotemporal properties of their receptive fields (RFs), and understanding these variations is key to elucidating how visual information is processed. We present a new approach for mapping RFs based on the filtered back projection (FBP), an algorithm used for tomographic reconstructions. To estimate RFs, a series of bars were flashed across the retina at pseudo-random positions and at a minimum of five orientations. We apply this method to retinal neurons and show that it can accurately recover the spatial RF and impulse response of ganglion cells recorded on a multi-electrode array. We also demonstrate its utility for in vivo imaging by mapping the RFs of an array of bipolar cell synapses expressing a genetically encoded Ca2+ indicator. We find that FBP offers several advantages over the commonly used spike-triggered average (STA): (i) ON and OFF components of a RF can be separated; (ii) the impulse response can be reconstructed at sample rates of 125 Hz, rather than the refresh rate of a monitor; (iii) FBP reveals the response properties of neurons that are not evident using STA, including those that display orientation selectivity, or fire at low mean spike rates; and (iv) the FBP method is fast, allowing the RFs of all the bipolar cell synaptic terminals in a field of view to be reconstructed in under 4 min. Use of the FBP will benefit investigations of the visual system that employ electrophysiology or optical reporters to measure activity across populations of neurons.
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影响因子：
16.2
作者：
Esposti F;Johnston J;Rosa JM;Leung KM;Lagnado L
通讯作者：
Lagnado L
影响因子：
64.8
作者：
Berry, MJ;Brivanlou, IH;Meister, M
通讯作者：
Meister, M
影响因子：
2.5
作者：
Fairhall, Adrienne L.;Burlingame, C. Andrew;Berry, Michael J., II
通讯作者：
Berry, Michael J., II
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.5
作者：
KANEKO, A
通讯作者：
KANEKO, A