Chronic in vivo stability assessment of carbon fiber microelectrode arrays.
Chronic in vivo stability assessment of carbon fiber microelectrode arrays.
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碳纤维微电极阵列的慢性体内稳定性评估。
DOI：
10.1088/1741-2560/13/6/066002
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发表时间：
2016-12
影响因子：
4
通讯作者：
Chestek CA
中科院分区：
文献类型：
--
作者：
Patel PR;Zhang H;Robbins MT;Nofar JB;Marshall SP;Kobylarek MJ;Kozai TD;Kotov NA;Chestek CA
单个碳纤维微电极可以记录急性和半慢性(∼1个月)植入物的单位活动。此外，还开发了插入16通道碳纤维微电极阵列的新方法。在评估这些阵列的体内长期生存能力之前，进行了加速浸泡试验，以确定最稳定的部位涂层材料。接下来，用碳纤维微电极阵列和硅电极进行了多动物、多月、慢性植入研究。碳纤维首先用两种不同配方的PEDOT中的一种进行功能化，然后在热水浴中进行加速老化。在确定最佳PEDOT配方后，将碳纤维阵列长期植入大鼠运动皮质。一些啮齿动物也被植入了单一的硅电极，而另一些则同时接受了两种电极。在研究结束时，对一组动物进行灌流，并将脑组织切片。组织切片进行星形胶质细胞、小胶质细胞和神经元染色。采用定性和定量相结合的方法评估局部反应。电生理记录显示，碳纤维检测单元活动至少3个月，平均幅度为∼200μV。组织学分析显示，碳纤维阵列具有最小或不存在的胶质瘢痕反应，对神经元密度没有不利影响。硅电极显示大量的神经胶质疤痕，影响了神经元数量。本研究验证了碳纤维微电极阵列作为一种慢性神经记录技术的应用。这些电极已经证明能够在3个月内检测到高幅度的单个单元，并显示出记录更长时间的潜力。此外，最小反应应无限期地保持稳定，因为免疫系统的任何反应都可能在12周后达到稳定状态。
Individual carbon fiber microelectrodes can record unit activity in both acute and semi-chronic (∼1 month) implants. Additionally, new methods have been developed to insert a 16 channel array of carbon fiber microelectrodes. Before assessing the in vivo long-term viability of these arrays, accelerated soak tests were carried out to determine the most stable site coating material. Next, a multi-animal, multi-month, chronic implantation study was carried out with carbon fiber microelectrode arrays and silicon electrodes. Carbon fibers were first functionalized with one of two different formulations of PEDOT and subjected to accelerated aging in a heated water bath. After determining the best PEDOT formula to use, carbon fiber arrays were chronically implanted in rat motor cortex. Some rodents were also implanted with a single silicon electrode, while others received both. At the end of the study a subset of animals were perfused and the brain tissue sliced. Tissue sections were stained for astrocytes, microglia, and neurons. The local reactive responses were assessed using qualitative and quantitative methods. Electrophysiology recordings showed the carbon fibers detecting unit activity for at least 3 months with average amplitudes of ∼200 μV. Histology analysis showed the carbon fiber arrays with a minimal to non-existent glial scarring response with no adverse effects on neuronal density. Silicon electrodes showed large glial scarring that impacted neuronal counts. This study has validated the use of carbon fiber microelectrode arrays as a chronic neural recording technology. These electrodes have demonstrated the ability to detect single units with high amplitude over 3 months, and show the potential to record for even longer periods. In addition, the minimal reactive response should hold stable indefinitely, as any response by the immune system may reach a steady state after 12 weeks.
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DOI：
10.3389/fneng.2010.00005
发表时间：
2010
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
Desai SA;Rolston JD;Guo L;Potter SM
通讯作者：
Potter SM
影响因子：
4
作者：
Chestek CA;Gilja V;Nuyujukian P;Foster JD;Fan JM;Kaufman MT;Churchland MM;Rivera-Alvidrez Z;Cunningham JP;Ryu SI;Shenoy KV
通讯作者：
Shenoy KV
基于层状硅的慢性植入平面硅探针的机械故障模式，用于层流记录。
影响因子：
14
作者：
Kozai, Takashi D. Y.;Catt, Kasey;Li, Xia;Gugel, Zhannetta V.;Olafsson, Valur T.;Vazquez, Alberto L.;Cui, X. Tracy
通讯作者：
Cui, X. Tracy
神经元型特异性信号，用于腹侧对段区域的奖励和惩罚。
影响因子：
64.8
作者：
Cohen, Jeremiah Y.;Haesler, Sebastian;Vong, Linh;Lowell, Bradford B.;Uchida, Naoshige
通讯作者：
Uchida, Naoshige
影响因子：
5
作者：
Kozai, Takashi D. Y.;Jaquins-Gerstl, Andrea S.;Vazquez, Alberto L.;Michael, Adrian C.;Cui, X. Tracy
通讯作者：
Cui, X. Tracy