In vivo comparison of biomimetic approaches for tissue regeneration of the scarred vocal fold.
In vivo comparison of biomimetic approaches for tissue regeneration of the scarred vocal fold.
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疤痕声带组织再生仿生方法的体内比较。
DOI：
10.1089/ten.tea.2008.0299
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发表时间：
2009
影响因子：
--
通讯作者：
Prestwich,Glenn
中科院分区：
文献类型：
--
作者：
Thibeault,SusanL;Klemuk,SarahA;Smith,MarshallE;Leugers,Cecilia;Prestwich,Glenn
本研究的目的是确定三种不同的仿生方法是否可以促进组织再生并改善疤痕声带固有层细胞外基质（ECM）的粘弹性特性。对20只兔子声带进行双侧活检；损伤后 2 个月，用 (i) 自体成纤维细胞、(ii) 半合成 ECM (sECM) 或 (iii) 封装在 sECM 中的自体成纤维细胞单侧治疗兔子。将盐水作为对照注射到对侧褶皱中。治疗后2个月处死动物。测量的结果是固有层中的前胶原、胶原蛋白和纤连蛋白水平，以及三个频率十年的组织粘度和弹性。所有治疗组均表现出 ECM 增殖加速。与盐水相比，用自体成纤维细胞处理的声带固有层被发现具有显着改善的粘度（p= 0.0077）和弹性（p= 0.0081）。该治疗组的纤连蛋白水平显着升高。 sECM 和自体成纤维细胞/sECM 组的前胶原、胶原和纤连蛋白水平显着升高，表明与具有粘弹性测量值的盐水相比，基质产生丰富，与对照组没有统计学差异。自体成纤维细胞的使用可以更好地恢复声带固有层的生物力学特性。细胞-支架相互作用和随后的细胞行为的优化对于支架和支架-细胞方法的利用是必要的。
The objective of this study was to determine if three different biomimetic approaches could facilitate tissue regeneration and improve viscoelastic properties in the scarred vocal fold lamina propria extracellular matrix (ECM). Twenty rabbit vocal folds were biopsied bilaterally; 2 months postinjury rabbits were unilaterally treated with (i) autologous fibroblasts, (ii) a semisynthetic ECM (sECM), or (iii) autologous fibroblasts encapsulated in sECM. Saline was injected as a control into the contralateral fold. Animals were sacrificed 2 months after treatment. Outcomes measured were procollagen, collagen, and fibronectin levels in the lamina propria, and tissue viscosity and elasticity across three frequency decades. All treatment groups demonstrated accelerated proliferation of the ECM. Vocal fold lamina propria treated with autologous fibroblasts were found to have significantly improved viscosity (p= 0.0077) and elasticity (p= 0.0081) compared to saline. This treatment group had significantly elevated fibronectin levels. sECM and autologous fibroblasts/sECM groups had significantly elevated levels of procollagen, collagen, and fibronectin, indicating abundant matrix production as compared to saline with viscoelastic measures that did not differ statistically from controls. The use of autologous fibroblasts led to better restoration of the vocal fold lamina propria biomechanical properties. Optimization of cell–scaffold interactions and subsequent cell behavior is necessary for utilization of scaffold and scaffold–cell approaches.
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影响因子：
2.2
作者：
Thibeault, SL;Gray, SD;Ford, CN
通讯作者：
Ford, CN
影响因子：
6.2
作者：
Shu, XZ;Liu, YC;Prestwich, GD
通讯作者：
Prestwich, GD
影响因子：
14
作者：
Liu, YC;Shu, XZ;Prestwich, GD
通讯作者：
Prestwich, GD
用于组织工程和修复的可注射合成细胞外基质。
影响因子：
--
作者：
Prestwich,GlennD;Shu,XiaoZheng;Liu,Yanchun;Cai,Shenshen;Walsh,JenniferF;Hughes,CaseyW;Ahmad,Shama;Kirker,KellyR;Yu,Bolan;Orlandi,RichardR;Park,AlbertH;Thibeault,SusanL;Duflo,Suzy;Smith,MarshallE
通讯作者：
Smith,MarshallE
制造参数对 2,3-二醛纤维素膜粘弹性剪切模量的影响——声带固有层组织工程的潜在支架。
DOI：
10.1002/jbm.a.31921
发表时间：
2009
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Roychowdhury,Priyanka;Klemuk,Sarah;Titze,Ingo;Kumar,Vijay
通讯作者：
Kumar,Vijay