Nanoparticle-based endodontic antimicrobial photodynamic therapy.
Nanoparticle-based endodontic antimicrobial photodynamic therapy.
复制标题
DOI：
10.1016/j.joen.2009.10.011
复制
发表时间：
2010-02
影响因子：
4.2
通讯作者：
Soukos NS
中科院分区：
文献类型：
--
作者：
Pagonis TC;Chen J;Fontana CR;Devalapally H;Ruggiero K;Song X;Foschi F;Dunham J;Skobe Z;Yamazaki H;Kent R;Tanner AC;Amiji MM;Soukos NS
目的：研究光敏剂亚甲基蓝(MB)与光复合的聚乳酸-乙醇酸纳米粒对粪肠球菌(ATCC 29212)的体外抗菌作用。在粪肠球菌悬浮液中加入与胶体金络合的PLGA，分别孵育2.5、5和10min后，用透射电子显微镜观察纳米粒在粪肠球菌体内的摄取和分布。粪肠球菌在浮游期和实验感染的人拔牙的根管中，用载MB的纳米颗粒致敏10分钟，然后暴露在665 nm的红光下。在所有三个时间点，纳米颗粒都被发现主要集中在微生物的细胞壁上。光和MB纳米颗粒的协同作用导致浮游期和根管中的菌落形成单位分别减少了约2log10和1log10。在这两种情况下，平均log10CFU水平显著低于对照组和没有光线的MB负载纳米颗粒。PLGA纳米粒包埋光活性药物有望成为抗菌牙髓治疗的一种有前景的辅助材料。
To study the in vitro effects of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the photosensitizer methylene blue (MB) and light against Enterococcus faecalis (ATCC 29212). The uptake and distribution of nanoparticles in E. faecalis in suspension was investigated by transmission electron microscopy (TEM) after incubation with PLGA complexed with colloidal gold particles for 2.5, 5 and 10 minutes. E. faecalis species were sensitized in planktonic phase and in experimentally infected root canals of human extracted teeth with MB-loaded nanoparticles for 10 minutes followed by exposure to red light at 665 nm. The nanoparticles were found to be concentrated mainly on the cell walls of microorganisms at all three time points. The synergism of light and MB-loaded nanoparticles led to approximately 2 and 1 log10 reduction of colony-forming units in planktonic phase and root canals, respectively. In both cases, mean log10 CFU levels were significantly lower than controls and MB-loaded nanoparticles without light. The utilization of PLGA nanoparticles encapsulated with photoactive drugs may be a promising adjunct in antimicrobial endodontic treatment.
登录
查看更多内容
影响因子：
4.2
作者：
Fimple, Jacob Lee;Fontana, Carla Raquel;Soukos, Nikolaos S.
通讯作者：
Soukos, Nikolaos S.
影响因子：
5.8
作者：
Jeong, Young-Il;Na, Hee-Sam;Nah, Jae-Woon
通讯作者：
Nah, Jae-Woon
影响因子：
4.2
作者：
Distel, JW;Hatton, JF;Gillespie, MJ
通讯作者：
Gillespie, MJ
影响因子：
3.5
作者：
Fontana CR;Abernethy AD;Som S;Ruggiero K;Doucette S;Marcantonio RC;Boussios CI;Kent R;Goodson JM;Tanner AC;Soukos NS
通讯作者：
Soukos NS
影响因子：
10.8
作者：
McCarthy, JR;Perez, JM;Weissleder, R
通讯作者：
Weissleder, R