WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
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DOI：
10.1016/j.ydbio.2013.03.012
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发表时间：
2013-05-15
影响因子：
2.7
通讯作者：
Soto, Martha C.
中科院分区：
文献类型：
--
作者：
Patel, Falshruti B.;Soto, Martha C.
细胞可以利用肌动蛋白聚合的力量来驱动细胞内运输，但肌动蛋白在内吞作用中的作用尚不清楚。在单细胞酵母中的研究证明了分支肌动蛋白成核剂Arp 2/3及其活化成核促进因子（NPFs）在通过胞吞作用从细胞表面内陷的过程中的重要作用。然而，一些哺乳动物研究对多细胞生物中网格蛋白介导的内吞作用（CME）中需要F-肌动蛋白和Arp 2/3存在争议。我们研究了Arp 2/3在C. elegans，一种具有极化上皮的多细胞生物。Arp 2/3及其NPF（WAVE/SCAR）是C.线虫胚胎形态发生我们发现WAVE/SCAR和Arp 2/3在不同的组织中调节胞吞作用和早期内体形态。优美的WAVE/SCAR或Arp 2/3的耗尽，而不是NPF Wasp的耗尽，严重破坏了通过CME内化的分子的分布，并改变了早期内体调节因子RAB-5的亚细胞富集。WAVE/SCAR或调节RAB-5的GEF的缺失导致肠和体腔细胞中内吞作用的类似缺陷。本研究在多细胞生物中支持分支肌动蛋白调节因子在内吞作用中的重要作用，并确定WAVE/SCAR是促进C.优雅的。
Cells can use the force of actin polymerization to drive intracellular transport, but the role of actin in endocytosis is not clear. Studies in single-celled yeast demonstrate the essential role of the branched actin nucleator, Arp2/3, and its activating nucleation promoting factors (NPFs) in the process of invagination from the cell surface through endocytosis. However, some mammalian studies have disputed the need for F-actin and Arp2/3 in Clathrin-Mediated Endocytosis (CME) in multicellular organisms. We investigate the role of Arp2/3 during endocytosis in C. elegans, a multicellular organism with polarized epithelia. Arp2/3 and its NPF, WAVE/SCAR, are essential for C. elegans embryonic morphogenesis. We show that WAVE/SCAR and Arp2/3 regulate endocytosis and early endosome morphology in diverse tissues of C. elegans. Depletion of WAVE/SCAR or Arp2/3, but not of the NPF Wasp, severely disrupts the distribution of molecules proposed to be internalized via CME, and alters the subcellular enrichment of the early endosome regulator RAB-5. Loss of WAVE/SCAR or of the GEFs that regulate RAB-5 results in similar defects in endocytosis in the intestine and coelomocyte cells. This study in a multicellular organism supports an essential role for branched actin regulators in endocytosis, and identifies WAVE/SCAR as a key NPF that promotes Arp2/3 endocytic function in C. elegans.
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影响因子：
21.3
作者：
通讯作者：
--
DOI：
10.1083/jcb.101.4.1532
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Francis GR;Waterston RH
通讯作者：
Waterston RH
影响因子：
21.3
作者：
Anitei, Mihaela;Stange, Christoph;Parshina, Irina;Baust, Thorsten;Schenck, Annette;Raposo, Graca;Kirchhausen, Tomas;Hoflack, Bernard
通讯作者：
Hoflack, Bernard
影响因子：
3.3
作者：
Hermann, GJ;Schroeder, LK;Priess, JR
通讯作者：
Priess, JR
影响因子：
9.2
作者：
Collins, Agnieszka;Warrington, Anthony;Taylor, Kenneth A.;Svitkina, Tatyana
通讯作者：
Svitkina, Tatyana