Genetic diversity and population structure of Plasmodium vivax in Central China.
Genetic diversity and population structure of Plasmodium vivax in Central China.
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华中地区间日疟原虫遗传多样性及种群结构
DOI：
10.1186/1475-2875-13-262
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发表时间：
2014-07-09
期刊：
影响因子：
3
通讯作者：
Gao Q
中科院分区：
文献类型：
--
作者：
Liu Y;Auburn S;Cao J;Trimarsanto H;Zhou H;Gray KA;Clark TG;Price RN;Cheng Q;Huang R;Gao Q
研究背景在华中地区，间日疟原虫发病率的下降被流行扩大和输入性病例所打断。这些变化对当地的寄生虫种群的影响，并并发的风险，未来的复苏，进行了assessment.MethodsPlasmodium间日疟原虫分离株收集从安徽省和江苏省，中国中部2007年至2010年之间，使用毛细管电泳在7个多态性短串联重复序列标记的基因型。结果安徽94株（4%）和江苏25株（12%）多克隆感染较为少见，2008 ~ 2010年合并后多克隆感染频率呈上升趋势（2 ~ 19%）。在这两个省和测试的年份中，人口多样性很高（HE = 0.8 - 0.85）。安徽和江苏之间的差异不大（F 'ST = 0.1）。在安徽和江苏两地均发现了几个具有相同多位点单倍型的菌株群。连锁不平衡在两个群体和每年测试（IAS = 0.2 - 0.4），但下降两至四倍时，相同的单倍型占，偶尔流行病传播动力学的指示。五个进口分离株共享相同的单倍型的任何中国中部isolations.ConclusionsThe人口间日疟原虫在中国中部的遗传结构突出了不稳定的传输，与有限的障碍，中部省份之间的基因流动。尽管地方性较低，但种群多样性仍然很高，但维持这种多样性的水库仍不清楚。输入性病例的挑战和死灰复燃的风险突出表明，需要继续监测，以发现早期预警信号。虽然寄生虫基因分型有可能为暴发管理提供信息，但需要进一步研究以确定合适的标记物组，用于分辨本地和进口间日疟原虫分离株。
BackgroundIn Central China the declining incidence of Plasmodium vivax has been interrupted by epidemic expansions and imported cases. The impact of these changes on the local parasite population, and concurrent risks of future resurgence, was assessed.MethodsPlasmodium vivax isolates collected from Anhui and Jiangsu provinces, Central China between 2007 and 2010 were genotyped using capillary electrophoresis at seven polymorphic short tandem repeat markers. Spatial and temporal analyses of within-host and population diversity, population structure, and relatedness were conducted on these isolates.ResultsPolyclonal infections were infrequent in the 94 isolates from Anhui (4%) and 25 from Jiangsu (12%), with a trend for increasing frequency from 2008 to 2010 (2 to 19%) when combined. Population diversity was high in both provinces and across the years tested (HE = 0.8 – 0.85). Differentiation between Anhui and Jiangsu was modest (F’ST = 0.1). Several clusters of isolates with identical multi-locus haplotypes were observed across both Anhui and Jiangsu. Linkage disequilibrium was strong in both populations and in each year tested (IAS = 0.2 – 0.4), but declined two- to four-fold when identical haplotypes were accounted for, indicative of occasional epidemic transmission dynamics. None of five imported isolates shared identical haplotypes to any of the central Chinese isolates.ConclusionsThe population genetic structure of P. vivax in Central China highlights unstable transmission, with limited barriers to gene flow between the central provinces. Despite low endemicity, population diversity remained high, but the reservoirs sustaining this diversity remain unclear. The challenge of imported cases and risks of resurgence emphasize the need for continued surveillance to detect early warning signals. Although parasite genotyping has potential to inform the management of outbreaks, further studies are required to identify suitable marker panels for resolving local from imported P. vivax isolates.
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影响因子：
3.7
作者：
Abdullah NR;Barber BE;William T;Norahmad NA;Satsu UR;Muniandy PK;Ismail Z;Grigg MJ;Jelip J;Piera K;von Seidlein L;Yeo TW;Anstey NM;Price RN;Auburn S
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影响因子：
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作者：
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通讯作者：
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