The impact of B-cell perturbations on pneumococcal conjugate vaccine response in HIV-infected adults.
The impact of B-cell perturbations on pneumococcal conjugate vaccine response in HIV-infected adults.
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DOI：
10.1371/journal.pone.0042307
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Erikstrup C
中科院分区：
文献类型：
--
作者：
Johannesson TG;Søgaard OS;Tolstrup M;Petersen MS;Bernth-Jensen JM;Østergaard L;Erikstrup C
未经治疗的艾滋病毒感染导致B细胞种群严重紊乱和对疫苗接种反应不足。我们研究了已治疗和未治疗的HIV患者循环B细胞亚群与肺炎球菌结合疫苗抗体反应之间的关系。95例HIV感染者根据抗逆转录病毒治疗(ART)和CD_4~+细胞计数分为三组：20例ART初治者(未接受抗逆转录病毒治疗)，62例ART应答者(接受ART治疗，CD_4细胞计数500个/µL)，13例反应受损者(接受ART治疗3年以上，CD_4计数500细胞/µL)。所有受试者在基线和3个月后分别接种含或不含1 mg CpG7909(Toll样受体9激动剂)的双价7价肺炎球菌结合疫苗2次。免疫前用流式细胞仪检测B细胞亚群。免疫后3、4、9个月用酶联免疫吸附试验测定血清中疫苗血清型的免疫球蛋白浓度。与受损者相比，ART反应者有更多的同型转换记忆B细胞和更多的边缘带(MZ)样B细胞。此外，ART幼稚患者的移行B细胞和浆母细胞浓度高于其他患者组。MZ样细胞、同型转换记忆细胞和浆母细胞的浓度与免疫后3、4、9个月的免疫球蛋白浓度呈正相关。低浓度的同型转换记忆B细胞是肺炎球菌结合疫苗反应差的最强独立预测因子，强调B细胞亚群紊乱与HIV感染患者的疫苗反应差有关
Untreated HIV infection results in severe perturbations of the B-cell population and hyporesponsiveness to vaccination. We studied associations between circulating B-cell subsets and antibody response to pneumococcal conjugate vaccine in treated and untreated HIV patients. Ninety-five HIV-infected adults were grouped according to antiretroviral therapy (ART) and CD4+ cell count as follows: 20 ART-naïve (no prior ART), 62 ART-responders (received ART, and CD4 count >500 cells/µl), and 13 impaired responders (received ART for more than 3 years, and CD4 count <500 cells/µl). All subjects were immunized twice with double-dose 7-valent pneumococcal conjugate vaccine with or without 1 mg CPG 7909 (toll-like receptor 9 agonist) at baseline and after three months. Pre-vaccination B-cell subpopulations were assessed by flow cytometry. Serum IgG concentrations for vaccine serotypes were quantified by ELISA at baseline and 3, 4, and 9 months post-vaccination. ART responders had more isotype-switched memory B cells and more marginal-zone (MZ)-like B cells compared with impaired responders. Furthermore, ART-naïve patients had higher concentration of transitional B cells and plasmablasts compared with B cells of other patient groups. The concentration of MZ-like, isotype switched memory cells and plasmablasts correlated positively with post-vaccination IgG concentration at 3, 4, and 9 months. Low concentrations of isotype-switched memory B cells was the strongest independent predictor of poor pneumococcal conjugate vaccine responsiveness, emphasizing that B-cell subset disturbances are associated with poor vaccine response among HIV-infected patients
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影响因子：
3.8
作者：
Barry, PM;Zetola, N;Lucas, GM
通讯作者：
Lucas, GM
DOI：
10.1038/nri2524
发表时间：
2009-04
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.8
作者：
Dworkin, MS;Ward, JW;Kaplan, JE
通讯作者：
Kaplan, JE
影响因子：
20.3
作者：
Moir, Susan;Buckner, Clarisa M.;Fauci, Anthony S.
通讯作者：
Fauci, Anthony S.
影响因子：
15.3
作者：
Morris, L;Binley, J M;Clas, B A;Bonhoeffer, S;Astill, T P;Kost, R;Hurley, A;Cao, Y;Markowitz, M;Ho, D D;Moore, J P
通讯作者：
Moore, J P