Influence of design and material characteristics on 3D printed flow-cells for heat transfer-based analytical devices.
Influence of design and material characteristics on 3D printed flow-cells for heat transfer-based analytical devices.
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DOI：
10.1007/s00604-022-05163-2
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发表时间：
2022-01-24
期刊：
影响因子：
--
通讯作者：
Peeters M
中科院分区：
文献类型：
--
作者：
Figueiredo LF;Vieira FS;Jamieson OD;Reeder J;Mc Lean T;Olsen J;Crapnell RD;Whittingham MJ;Banks CE;Law R;Gruber J;Peeters M
据报道，重新设计的3D打印流动池用于基于传热学的生物分子检测，从流过系统到加入型测量池。本研究的目的是评估这种新的测量设计的性能，并批判性地分析材料特性和3D打印方法对热分析的影响。为了使该技术适合临床应用，特别注意减少稳定时间、样本量，并通过减少气泡的噪声和干扰来提高平台的灵敏度。研究了三种不同的方法，包括只使用熔丝制造(FFF)的丝状聚乳酸电池，使用立体曝光(SLA)印刷的树脂电池，以及通过金属注射成型(MIM)和熔丝制造(FFF)相结合的铜制成的设计。计算流体动力学(CFD)建模使用了Ansys Fluent V18.1，以深入了解测量单元内的热量流动，从而促进系统和理论响应速度的优化。结果表明，使用SLA的测量细胞具有最低的噪声(~ 0.6%)和最短的测量时间(15min)，而使用其他方法产生的测量细胞的特异性较低或存在排尿问题。最后，我们评估了这些新设计在检测生物分子和阿莫西林(一种常用的β-内酰胺类抗生素)方面的潜力，以证明这一概念的有效性。由此可见，用SLA生产的树脂加入型测定池是一种有趣的、负担得起的替代方法，能够以高灵敏度检测阿莫西林，由于测定池的一次性性质以及样品体积小，因此具有很大的临床应用前景。网上版载有补充材料，可在10.1007/s00604-022-05163-2查阅。
Redesigning 3D-printed flow cells is reported used for heat transfer based detection of biomolecules from a flow-through system to an addition-type measurement cell. The aim of this study is to assess the performance of this new measurement design and critically analyse the influence of material properties and 3D printing approach on thermal analysis. Particular attention is paid to reduce the time to stabilisation, the sample volume in order to make the technique suitable for clinical applications, and improving the sensitivity of the platform by decreasing the noise and interference of air bubbles. The three different approaches that were studied included a filament polylactic acid cell using only fused filament fabrication (FFF), a resin cell printed using stereolitography (SLA), and finally a design made of copper, which was manufactured by combining metal injection moulding (MIM) with fused filament fabrication (FFF). Computational fluid dynamic (CFD) modelling was undertaken using ANSYS Fluent V18.1 to provide insight into the flow of heat within the measurement cell, facilitating optimisation of the system and theoretical response speed. It was shown that the measurement cells using SLA had the lowest noise (~ 0.6%) and shortest measurement time (15 min), whereas measurement cells produced using other approaches had lower specificity or suffered from voiding issues. Finally, we assessed the potential of these new designs for detection of biomolecules and amoxicillin, a commonly used beta lactam antibiotic, to demonstrate the proof of concept. It can be concluded that the resin addition-type measurement cells produced with SLA are an interesting affordable alternative, which were able to detect amoxicillin with high sensitivity and have great promise for clinical applications due to the disposable nature of the measurement cells in addition to small sample volumes. The online version contains supplementary material available at 10.1007/s00604-022-05163-2.
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DOI：
10.3390/s130709148
发表时间：
2013-07-16
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Geerets B;Peeters M;van Grinsven B;Bers K;de Ceuninck W;Wagner P
通讯作者：
Wagner P
影响因子：
9.5
作者：
van Grinsven, Bart;Eersels, Kasper;Wagner, Patrick
通讯作者：
Wagner, Patrick
影响因子：
17.1
作者：
van Grinsven, Bart;Vanden Bon, Natalie;Wagner, Patrick
通讯作者：
Wagner, Patrick
影响因子：
64.8
作者：
VLATAKIS, G;ANDERSSON, LI;MOSBACH, K
通讯作者：
MOSBACH, K
DOI：
10.1002/pssa.201600758
发表时间：
2017-09-01
影响因子：
2
作者：
Stilman, Wouter;Jooken, Stijn;Eersels, Kasper
通讯作者：
Eersels, Kasper