Mining and expression analysis of candidate genes involved in regulating the chilling requirement fulfillment of Paeonia lactiflora 'Hang Baishao'.
Mining and expression analysis of candidate genes involved in regulating the chilling requirement fulfillment of Paeonia lactiflora 'Hang Baishao'.
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芍药‘杭白芍’耐冷需求调控候选基因挖掘及表达分析
DOI：
10.1186/s12870-017-1205-1
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发表时间：
2017-12-22
影响因子：
5.3
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Zhang J;Li D;Shi X;Zhang D;Qiu S;Wei J;Zhang J;Zhou J;Zhu K;Xia Y
人工扩大观赏植物的种植面积和生态幅度，在园艺和园林应用中具有重要意义。芍药（学名：Acanthonia lactiflora Pall.）是一种世界著名的观赏植物，花形艳丽、芳香，主要种植于温带和冷凉地区。亚热带和热带北方半球冬季气温偏高，导致牡丹芽休眠解除所需的冷量积累不足，严重阻碍了“南种植园”。因此，有必要对牡丹的休眠性、需冷量及其相关分子机制进行深入研究，以扩大牡丹这一珍贵园艺品种的应用范围。根据自然和人工降温试验以及降温时间（CH）和降温单位（CU）评价体系，“杭白哨”的最低降温时间（CR）在504.00 ~ 672.00CHs之间，最佳降温时间（CR）为672.00CHs和856.08CU，以获得较强的发芽、生长和开花性能。以杭白韶休眠期和萌发期的芽为材料，进行转录组测序和RNA-Seq基因鉴定。构建了6个基因文库，并确定了66个温度和光周期相关的unigenes作为可能调控或可能决定CR特性的潜在候选基因。2012-2013年冬季和2015-2016年冬季之间的表达模式的差异，以及这两个冬季之间的CR实现期的差异，代表了有趣的一致性关系。WRKY DNA结合蛋白33（WRKY 33）也观察到这种相关性。综合各试验结果，‘杭白韶’是一个具有显著低CR性状的优良牡丹资源。SOC 1和WRKY 33两个基因可能参与决定杭白菊的CR量和完成期。热休克蛋白、骨促动素和CAB表达的时间也值得关注。本研究有助于了解影响CR特性的深层因素和机制，为亚热带地区培育低CR的园林或园艺应用新种质提供参考。本文的在线版本（10.1186/s12870-017-1205-1）包含补充材料，可供授权用户使用。
The artificial enlargement of the planting area and ecological amplitude of ornamentals for horticultural and landscape applications are significant. Herbaceous peony (Paeonia lactiflora Pall.) is a world-famous ornamental with attractive and fragrant flowers and is mainly planted in temperate and cool areas. Comparatively higher winter temperatures in the subtropical and tropical Northern Hemisphere result in a deficit of chilling accumulation for bud dormancy release, which severely hinders “The southward plantation of herbaceous peony”. Studies on the dormancy, chilling requirement (CR) and relevant molecular mechanisms of peony are needed to enhance our ability to extend the range of this valuable horticultural species. Based on natural and artificial chilling experiments, and chilling hour (CH) and chilling unit (CU) evaluation systems, the lowest CR of ‘Hang Baishao’ was between 504.00 and 672.00 CHs and the optimal CR was 672.00 CHs and 856.08 CUs for achieving strong sprouting, growth and flowering performance. Transcriptome sequencing and gene identification by RNA-Seq were performed on ‘Hang Baishao’ buds during the dormancy and sprouting periods. Six gene libraries were constructed, and 66 temperature- and photoperiod-associated unigenes were identified as the potential candidate genes that may regulate or possibly determine CR characteristics. The difference in the expression patterns of SUPPRESSPOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) between the winters of 2012–2013 and 2015–2016, and the difference of CR fulfillment periods also between these two winters represented the interesting congruent relationships. This correlation was also observed for WRKY DNA-BINDING PROTEIN 33 (WRKY 33). Combined with the results acquired from all of experiments, ‘Hang Baishao’ was confirmed to be a superb peony resource that have significantly low CR characteristics. The two genes of SOC1 and WRKY33 are likely involved in determining the CR amount and fulfillment period of ‘Hang Baishao’. HEAT SHOCK PROTEIN, OSMOTIN and TIMING OF CAB EXPRESSION 1 also deserve attention for the CR research. This study could contribute to the knowledge of the deep factors and mechanisms that regulate CR characteristics, and may be beneficial for breeding new germplasms that have low CRs for landscape or horticulture applications in subtropical regions. The online version of this article (10.1186/s12870-017-1205-1) contains supplementary material, which is available to authorized users.
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综合转录组分析揭示了茶树腋芽在旁休眠、内休眠、生态休眠和芽盛期的差异基因表达谱
影响因子：
5.6
作者：
Hao X;Yang Y;Yue C;Wang L;Horvath DP;Wang X
通讯作者：
Wang X
影响因子：
7.4
作者：
Alter, Philipp;Bircheneder, Susanne;Dresselhaus, Thomas
通讯作者：
Dresselhaus, Thomas
影响因子：
5.1
作者：
Horvath, David P.;Sung, Sibum;Anderson, James
通讯作者：
Anderson, James
影响因子：
4.3
作者：
Hall, A. J.;Catley, J. L.;Walton, E. F.
通讯作者：
Walton, E. F.
影响因子：
5.6
作者：
Chowdhury S;Basu A;Kundu S
通讯作者：
Kundu S