Complete genomic sequence and analysis of β2 toxin gene mapping of Clostridium perfringens JXJA17 isolated from piglets in China.
Complete genomic sequence and analysis of β2 toxin gene mapping of Clostridium perfringens JXJA17 isolated from piglets in China.
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中国仔猪产气荚膜梭菌JXJA17全基因组序列及β2毒素基因图谱分析
DOI：
10.1038/s41598-020-79333-8
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发表时间：
2021-01-12
影响因子：
4.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zeng X;Liu B;Zhou J;Dai Y;Han C;Wang L;Wu Y;Zhang J
产气荚膜梭菌（Clostridium perfringens，Cp）是一种在自然环境和动物肠道中普遍存在的人畜机会致病菌。Cp的致病性取决于染色体或质粒上的基因编码的毒素的产生。在当代文献中，关于CpA β2毒素的致病性没有明确的共识。为了分析仔猪源CpA及其β2毒素基因组的同源性，采用Illumina Miseq和Pacbio Sequel平台对从腹泻仔猪中分离的JXJA 17菌株进行全基因组测序。基因组由一条环状染色体（3，324，072 bp，G + C含量为28.51%）和9个质粒组成。基因组和16 SrDNA同源性分析表明，JXJA 17株与JGS 1495、Cp-06、Cp-16和FORC_003株亲缘关系较近。这些菌株分离自不同的样品，属于不同的毒素类型。JXJA 17菌株携带两种毒素基因（plc和cpb 2）。与其它Cp株相比，JXJA 17的cpb 2基因位于一个大质粒（58 kb）上，没有其它毒素基因或抗生素抗性基因的共定位。分析JXJA 17基因组同源性及其cpb 2基因，为进一步研究β2毒素与仔猪腹泻的关系奠定基础。
Clostridium perfringens (Cp) is a ubiquitous opportunistic pathogen of humans and animals in the natural environment and animal intestines. The pathogenicity of Cp depends on the production of toxins encoded by genes on the chromosomes or plasmids. In contemporary literature, there is no clear consensus about the pathogenicity of CpA β2 toxin. To analyze the homology of the genome of piglet source CpA and its β2 toxin, we sequenced the whole genome of strain JXJA17 isolated from diarrhea piglets using the Illumina Miseq and Pacbio Sequel platforms. The genome was composed of a circular chromosome with 3,324,072 bp (G + C content: 28.51%) and nine plasmids. Genome and 16S rDNA homology analysis revealed a close relation of the JXJA17 strain with the JGS1495, Cp-06, Cp-16, and FORC_003 strains. These strains were isolated from different samples and belonged to different toxin-types. JXJA17 strain was found to carry two toxin genes (plc and cpb2). In contrast to other Cp strains, the cpb2 of JXJA17 was located on a large plasmid (58 kb) with no co-localization of other toxin genes or antibiotic resistance genes. Analysis of JXJA17 genome homology and its cpb2 would facilitate our further study the relationship between β2 toxin and piglet diarrhea.
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影响因子：
4.6
作者：
Moreira GM;Salvarani FM;da Cunha CE;Mendonça M;Moreira ÂN;Gonçalves LA;Pires PS;Lobato FC;Conceição FR
通讯作者：
Conceição FR
影响因子：
4.2
作者：
Li C;Yan X;Lillehoj HS
通讯作者：
Lillehoj HS
影响因子：
3
作者：
Bhushan, Ashish;Mukherjee, Tanmoy;Kalia, Vipin Chandra
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影响因子：
2.3
作者：
Rood JI;Adams V;Lacey J;Lyras D;McClane BA;Melville SB;Moore RJ;Popoff MR;Sarker MR;Songer JG;Uzal FA;Van Immerseel F
通讯作者：
Van Immerseel F
影响因子：
4.4
作者：
Kalia VC;Mukherjee T;Bhushan A;Joshi J;Shankar P;Huma N
通讯作者：
Huma N