Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles.
Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles.
复制标题
使用MSP10寡核苷酸和金纳米颗粒对尿液中疟原虫的比色检测。
DOI：
10.1371/journal.pntd.0005029
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发表时间：
2016-10
影响因子：
3.8
通讯作者：
Gilman RH
中科院分区：
文献类型：
--
作者：
Alnasser Y;Ferradas C;Clark T;Calderon M;Gurbillon A;Gamboa D;McKakpo US;Quakyi IA;Bosompem KM;Sullivan DJ;Vinetz JM;Gilman RH
间日疟原虫是世界上最普遍的人类疟疾病因，可导致严重疾病，复发的可能性很高。它的遗传和地理多样性使其难以控制。间日疟原虫的研究不足，为了在流行地区控制疟疾，需要一种快速、准确和简单的诊断工具。在这项初步研究中，我们发现一种利用尿液中AuNPs和MSP10 DNA检测的比色系统可以快速、简便、廉价地鉴定间日疟原虫。该检测具有良好的敏感性（84%），高特异性（97%），与恶性疟原虫仅轻度交叉反应（21%）。它使用简单，具有可见的颜色变化，不需要光谱仪，使其适合在恶劣条件下使用。使用尿液消除了手指刺破的需要，增加了安全性和患者对这种模式的接受度。为了控制疟疾，迫切需要应用创新的诊断方法和新技术。纳米粒子可以在寄生虫水平较低的情况下增强疟疾的检测，同时提供快速和简单的方法。利用MSP10和金纳米颗粒鉴定尿液中间日疟原虫DNA的新方法可作为具有全球应用潜力的筛选工具。拟议的测试可能影响在资源匮乏环境中控制最常见的疟疾种类，因为它可以提供一种简单、快速、廉价和易于解释的测试。此外，使用尿液代替血液消除了针刺手指的需要，这将提高安全性，并可能提高大规模筛查计划的参与率。
Plasmodium vivax is the most prevalent cause of human malaria in the world and can lead to severe disease with high potential for relapse. Its genetic and geographic diversities make it challenging to control. P. vivax is understudied and to achieve control of malaria in endemic areas, a rapid, accurate, and simple diagnostic tool is necessary. In this pilot study, we found that a colorimetric system using AuNPs and MSP10 DNA detection in urine can provide fast, easy, and inexpensive identification of P. vivax. The test exhibited promising sensitivity (84%), high specificity (97%), and only mild cross-reactivity with P. falciparum (21%). It is simple to use, with a visible color change that negates the need for a spectrometer, making it suitable for use in austere conditions. Using urine eliminates the need for finger-prick, increasing both the safety profile and patient acceptance of this model. To control malaria, there is an urgent need for applying innovative diagnostics and new technologies. Nanoparticles can augment detection of malaria at lower parasite levels while providing fast and simple methodology. Novel use of MSP10 and gold nanoparticles to identify Plasmodium vivax’s DNA in urine can be utilized as screening tool with global application potentials. The proposed test could impact the control of the most common species of malaria in low resource settings as it could present a simple, fast, cheap and easy to interpret test. Furthermore, utilizing urine instead of blood eliminates the need for finger-prick which would increase safety profile and likely increase participation rate in mass screening programs.
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影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
12
作者：
Erdman, Laura K;Kain, Kevin C
通讯作者：
Kain, Kevin C
使用金纳米探针直接检测大肠杆菌基因组 DNA。
影响因子：
10.2
作者：
Padmavathy B;Vinoth Kumar R;Jaffar Ali BM
通讯作者：
Jaffar Ali BM
影响因子：
3.8
作者：
Guerra CA;Howes RE;Patil AP;Gething PW;Van Boeckel TP;Temperley WH;Kabaria CW;Tatem AJ;Manh BH;Elyazar IR;Baird JK;Snow RW;Hay SI
通讯作者：
Hay SI
影响因子：
2.9
作者：
Jeon, Weejeong;Lee, Seonghwan;Ban, Changill
通讯作者：
Ban, Changill