A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species.
A highly antigenic fragment within the zoonotic Cryptosporidium parvum Gp900 glycoprotein (Domain 3) is absent in human restricted Cryptosporidium species.
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DOI：
10.1371/journal.pone.0287997
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
我们鉴定了人畜共患寄生虫隐孢子虫gp900的免疫优势孢子体表面糖蛋白的片段（结构域3-D3），该片段不存在人源隐孢子虫和人源隐孢子虫。该片段具有高度的抗原性，能够有效区分人畜共患的细小C.和优先感染人类的物种/基因型。D3检测提供了一种血清学工具来确定人类隐孢子虫病的来源是动物还是人类。我们在实验挑战仔猪，小鼠，大鼠和羊驼中证明了这一点。我们推测，该片段的缺失可能在人原疟原虫和人细小疟原虫的gp900蛋白中发挥了关键作用。
We identified a fragment (Domain 3—D3) of the immunodominant sporozoite surface glycoprotein of the zoonotic parasite Cryptosporidium gp900, which is absent C. hominis and C. parvum anthroponosum. The fragment is highly antigenic and is able to effectively differentiate between zoonotic C. parvum and species/genotypes that infect preferentially humans. D3 detection provides a serological tool to determine whether the source of human cryptosporidiosis is of animal or human origin. We demonstrate this in experimentally challenged piglets, mice, rats, and alpaca. We speculate that the absence of this fragment from the C. hominis and C. parvum anthroponosum gp900 protein may play a key role in their host restriction.
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影响因子：
2.1
作者：
Ren, Xupeng;Zhao, Jinfeng;Xiao, Lihua
通讯作者：
Xiao, Lihua
影响因子：
1.3
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通讯作者：
Dubey, JP
影响因子：
11.8
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Li N;Xiao L;Alderisio K;Elwin K;Cebelinski E;Chalmers R;Santin M;Fayer R;Kvac M;Ryan U;Sak B;Stanko M;Guo Y;Wang L;Zhang L;Cai J;Roellig D;Feng Y
通讯作者：
Feng Y
影响因子：
4.9
作者：
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通讯作者：
Caccio, Simone M.
影响因子：
2.6
作者：
Fayer, Ronald;Santin, Monica;Trout, James M.
通讯作者：
Trout, James M.