Genetic and epigenetic variations induced by wheat-rye 2R and 5R monosomic addition lines.
Genetic and epigenetic variations induced by wheat-rye 2R and 5R monosomic addition lines.
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小麦-黑麦 2R 和 5R 单体加成系诱导的遗传和表观遗传变异
DOI：
10.1371/journal.pone.0054057
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tang Z
中科院分区：
文献类型：
--
作者：
Fu S;Sun C;Yang M;Fei Y;Tan F;Yan B;Ren Z;Tang Z
背景单体异附加系（Monosomic alien addition lines，MAALs）能诱导染色体结构变异，已被广泛应用于作物育种中，但其是否能引起显著的遗传和表观遗传变异尚不清楚。方法/主要发现在本研究中，小麦-黑麦2 R和5 R MAAL及其自交后代和亲本普通小麦通过扩增片段长度多态性（AFLP）和甲基化敏感扩增多态性（MSAP）分析。不同世代的MAAL表现出不同的遗传变异。一些后代，只含有42条小麦染色体表现出很大的遗传/表观遗传变异。隐性黑麦染色质已渗入小麦基因组。然而，其中一个含有隐性黑麦染色质的后代没有表现出突出的遗传/表观遗传变异。从改变的AFLP和MSAP条带中分别克隆到78个和49个序列。Blaundice搜索结果表明，几乎一半的序列与已知序列没有显著的相似性。反转录转座子主要参与遗传和表观遗传变异。遗传变异基本上影响了Gypsy类逆转录转座子，而表观遗传改变同样影响了Copia类和Gypsy类逆转录转座子。遗传和表观遗传变异很少影响低拷贝编码DNA序列。本研究结果为小麦-黑麦单体异附加系诱导不同的、剧烈的遗传/表观遗传变异提供了直接证据，这些变异可能不是由黑麦染色质渗入小麦引起的。因此，MAALs可直接作为拓宽普通小麦遗传多样性的有效手段。
Background Monosomic alien addition lines (MAALs) can easily induce structural variation of chromosomes and have been used in crop breeding; however, it is unclear whether MAALs will induce drastic genetic and epigenetic alterations. Methodology/Principal Findings In the present study, wheat-rye 2R and 5R MAALs together with their selfed progeny and parental common wheat were investigated through amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) analyses. The MAALs in different generations displayed different genetic variations. Some progeny that only contained 42 wheat chromosomes showed great genetic/epigenetic alterations. Cryptic rye chromatin has introgressed into the wheat genome. However, one of the progeny that contained cryptic rye chromatin did not display outstanding genetic/epigenetic variation. 78 and 49 sequences were cloned from changed AFLP and MSAP bands, respectively. Blastn search indicated that almost half of them showed no significant similarity to known sequences. Retrotransposons were mainly involved in genetic and epigenetic variations. Genetic variations basically affected Gypsy-like retrotransposons, whereas epigenetic alterations affected Copia-like and Gypsy-like retrotransposons equally. Genetic and epigenetic variations seldom affected low-copy coding DNA sequences. Conclusions/Significance The results in the present study provided direct evidence to illustrate that monosomic wheat-rye addition lines could induce different and drastic genetic/epigenetic variations and these variations might not be caused by introgression of rye chromatins into wheat. Therefore, MAALs may be directly used as an effective means to broaden the genetic diversity of common wheat.
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影响因子：
2.4
作者：
Szakacs, E.;Molnar-Lang, M.
通讯作者：
Molnar-Lang, M.
影响因子：
5.4
作者：
van Heusden, AW;Shigyo, M;Kik, C
通讯作者：
Kik, C
影响因子：
3.1
作者：
Ma, Xue-Feng;Gustafson, J. Perry
通讯作者：
Gustafson, J. Perry
小麦-黑麦双倍体揭示的串联重复序列、调控元件和启动子区域的变异。
影响因子：
3.1
作者：
Tang, Zong-Xiang;Fu, Shu-Lan;Zhang, Huai-Qiong
通讯作者：
Zhang, Huai-Qiong
影响因子：
5.4
作者：
Bento, Miguel;Gustafson, Perry;Silva, Manuela
通讯作者：
Silva, Manuela