Modulation of the levels of cytochromes P450 in rat liver and lung by dietary lipid.
Modulation of the levels of cytochromes P450 in rat liver and lung by dietary lipid.
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饮食脂质对大鼠肝脏和肺中细胞色素 P450 水平的调节。
DOI：
10.1016/0006-2952(92)90141-5
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发表时间：
1992
影响因子：
5.8
通讯作者：
Yang,CS
中科院分区：
文献类型：
--
作者：
Yoo,JS;Smith,TJ;Ning,SM;Lee,MJ;Thomas,PE;Yang,CS
本研究旨在探讨日粮脂类对几种P450同工酶的调节作用。雄性SD大鼠体重130~140g，禁食2天后，分别饲喂20%玉米油(CO)和脱脂(FF)饲料，连续4天。用肝微粒体测定了P450s 1A1和2、2A1、2B1和2、2C11、2E1和3A的催化活性和免疫化学可检测的蛋白水平。饲喂20%CO饲料的大鼠的微粒体中N-亚硝酸二甲胺脱甲基酶活性和P450 2E1蛋白的水平是饲喂FF饲料的大鼠的2倍。CO组6β-羟化睾酮和P4503A蛋白的水平也是FF组的2.5倍。相反，CO饮食不影响P4502C11蛋白的免疫检测水平及其催化活性，如苯丙胺脱甲基酶活性和睾酮2α羟化。P4501A1在两组中均未检测到，但1A2在CO组是FF组的2.5倍。在肝脏中，根据戊氧基间苯二酚脱烷基酶活性和蛋白质水平，两组大鼠肝脏中P4502B1的水平都很低，而CO饮食组的2B2水平是对照组的2.5倍。在饲喂不同剂量CO的大鼠肺微粒体中，P4502B1水平与饲料中CO水平呈负相关。结果表明，P450同工酶的组成水平受饲料脂肪的选择性调节，肝脏P450同工酶1A2、2B2、2E1和3A的含量受饲料脂肪的正向调节，而肺P450 2B1的含量受饲料脂肪的抑制。
This study was undertaken to investigate the effect of dietary lipid on the regulation of several constitutive P450 isozymes. Male Sprague-Dawley rats with body weights of 130–140 g were fed either a 20% corn oil (CO) diet or a fat-free (FF) diet for 4 days following 2 days of fasting. Using liver microsomes, the catalytic activities and immunochemically detectable protein levels of P450s 1A1 and 2, 2A1, 2B1 and 2, 2C11, 2E1, and 3A were determined. The microsomes from rats fed the 20% CO diet exhibited 2-fold higher levels inN-nitrosodimethylamine demethylase activity and P450 2E1 protein than those from rats fed the FF diet. the CO group also showed 2.5-fold higher levels in 6β-hydroxylation of testosterone and P450 3A protein than the FF group. In contrast, the CO diet did not affect the immunodetectable level of P450 2C11 protein and its catalytic activities such as benzphetamine demethylase activity and 2α-hydroxylation of testosterone. P450 1A1 was not detectable in either group, but 1A2 was 2.5-fold higher in the CO group than in the FF group. In the liver, the P450 2B1 level was very low in both groups as measured by pentoxyresorufin dealkylase activity and the protein level, whereas 2B2 was 2.5-fold higher in the CO diet group. In lung microsomes from rats fed different amounts of CO, an inverse relationship was observed between the P450 2B1 level and the dietary CO level. the results suggest that the constitutive levels of P450 isozymes are modulateted by dietary lipid in a selective manner; the levels of hepatic P450s 1A2, 2B2, 2E1, and 3A were regulated positively but the level of pulmonary P450 2B1 was suppressed by dietary lipid.
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饮食对混合功能氧化酶系统药物代谢的影响。
影响因子：
13.5
作者：
Yang,CS;Yoo,JS
通讯作者：
Yoo,JS
与细胞色素 P-450b 和 P-450e 同源的大鼠 mRNA 的组织特异性表达。
影响因子：
14.9
作者：
Omiecinski,CJ
通讯作者：
Omiecinski,CJ
DOI：
--
发表时间：
1977
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
C. S. Yang;F. S. Strickhart;L. P. Kicha
通讯作者：
L. P. Kicha
DOI：
10.1093/jn/121.7.959
发表时间：
1991-07
期刊：
The Journal of nutrition
影响因子：
--
作者：
Jeong-Sook H. Yoo;S. Ning;C. Pantuck;C. Pantuck;E. Pantuck;E. Pantuck;Chung S. Yang
通讯作者：
Jeong-Sook H. Yoo;S. Ning;C. Pantuck;C. Pantuck;E. Pantuck;E. Pantuck;Chung S. Yang
影响因子：
4.1
作者：
NASH, T
通讯作者：
NASH, T