Complementary effects of two growth factors in multifunctionalized silk nanofibers for nerve reconstruction.
Complementary effects of two growth factors in multifunctionalized silk nanofibers for nerve reconstruction.
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DOI：
10.1371/journal.pone.0109770
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Egles C
中科院分区：
文献类型：
--
作者：
Dinis TM;Vidal G;Jose RR;Vigneron P;Bresson D;Fitzpatrick V;Marin F;Kaplan DL;Egles C
为了形成周围神经再生的生物活性导向器，将丝素蛋白电纺成取向的纳米纤维。在电纺过程中，通过加入神经生长因子(NGF)和睫状神经营养因子(CNTF)对这些纤维进行功能化处理。生长在纤维上的PC12细胞证实了NGF的生物利用度和生物活性，而NGF没有从纤维中显著释放。大鼠背根神经节(DRGs)的原代神经元生长在纳米纤维上，并固定在纤维上，根据纤维的方向定向生长，生长锥形态也证实了这一点。这些生物功能化的纳米纤维导致它们接触处的轴突长度增加了3倍，这可能是由于NGF。在功能化的纳米纤维中，CNTF可以刺激胶质细胞的生长、排列和迁移。大鼠胚胎背根节的器官培养证实了多功能纳米纤维中这两种生长因子的互补作用，这使得胶质细胞在类似于原位发现的“邦纳带”的结构中迁移、排列和平行生长轴突。基于生物功能化排列的丝素纳米纤维的可移植多通道管道被开发为一种有组织的三维支架。因此，我们的生物活性丝管为生物和生物相容的神经引导管道提供了新的选择。
With the aim of forming bioactive guides for peripheral nerve regeneration, silk fibroin was electrospun to obtain aligned nanofibers. These fibers were functionalized by incorporating Nerve Growth Factor (NGF) and Ciliary NeuroTrophic Factor (CNTF) during electrospinning. PC12 cells grown on the fibers confirmed the bioavailability and bioactivity of the NGF, which was not significantly released from the fibers. Primary neurons from rat dorsal root ganglia (DRGs) were grown on the nanofibers and anchored to the fibers and grew in a directional fashion based on the fiber orientation, and as confirmed by growth cone morphology. These biofunctionalized nanofibers led to a 3-fold increase in neurite length at their contact, which was likely due to the NGF. Glial cell growth, alignment and migration were stimulated by the CNTF in the functionalized nanofibers. Organotypic culture of rat fetal DRGs confirmed the complementary effect of both growth factors in multifunctionalized nanofibers, which allowed glial cell migration, alignment and parallel axonal growth in structures resembling the ‘bands of Bungner’ found in situ. Graftable multi-channel conduits based on biofunctionalized aligned silk nanofibers were developed as an organized 3D scaffold. Our bioactive silk tubes thus represent new options for a biological and biocompatible nerve guidance conduit.
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影响因子：
6.2
作者：
Jin, HJ;Fridrikh, SV;Kaplan, DL
通讯作者：
Kaplan, DL
影响因子：
14
作者：
Bozkurt, Ahmet;Deumens, Ronald;Pallua, Norbert
通讯作者：
Pallua, Norbert
影响因子：
3.9
作者：
Lee JY;Bashur CA;Milroy CA;Forciniti L;Goldstein AS;Schmidt CE
通讯作者：
Schmidt CE
影响因子：
14
作者：
Altman, GH;Diaz, F;Kaplan, DL
通讯作者：
Kaplan, DL
DOI：
10.1073/pnas.0914456107
发表时间：
2010-04-06
影响因子：
11.1
作者：
Brunetti, V.;Maiorano, G.;Pompa, P. P.
通讯作者：
Pompa, P. P.