Overexpression of two cold-responsive ATAF-like NAC transcription factors from fine-stem stylo (Stylosanthes guianensis var. intermedia) enhances cold tolerance in tobacco plants
Overexpression of two cold-responsive ATAF-like NAC transcription factors from fine-stem stylo (Stylosanthes guianensis var. intermedia) enhances cold tolerance in tobacco plants
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细茎花柱 (Stylosanthes guianensis var. intermedia) 中两种冷响应 ATAF 样 NAC 转录因子的过表达可增强烟草植物的耐冷性
DOI：
10.1007/s11240-018-1486-6
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Xin‑Ming Xie
中科院分区：
文献类型：
--
作者：
Peng‑Lin Zhan;Shan‑Wen Ke;Pan‑Yu Zhang;Cong‑Cong Zhou;Bei‑Ling Fu;Xiang‑Qian Zhang;Tian‑Xiu Zhong;Shu Chen;Xin‑Ming Xie
柱花草是重要的热带、亚热带豆科牧草。它们易受寒冷和霜冻的影响，对它们响应或适应低温胁迫的遗传和分子机制知之甚少。从细茎柱花草（S. stylo）的全转录组分析中选择了两个冷响应NAC基因SgNAC 1和SgNAC 2。圭亚那变种intermedia），并进一步研究了它们在冷胁迫耐受性中的作用。生物信息学分析表明，SgNAC1和SgNAC2属于NAC家族的ATAF亚类，与ATAF样NAC转录因子具有高度保守的N端A-E NAC亚结构域和EVQS [E/x] PK [W/I]基序。表达谱分析、亚细胞定位和反式激活试验表明，SgNAC1和SgNAC2编码具有反式激活活性的核定位多肽，并迅速响应冷胁迫。表型和生理变化表明，转基因烟草比野生型植株更耐冷胁迫。同时，SgNAC1和SgNAC2的表达在低温处理的早期显著增强，表明SgNAC1和SgNAC2的过表达足以赋予烟草植物抗寒性。这些结果表明，SgNAC1和SgNAC2是柱花草耐冷性改良策略的有希望的候选基因。
Stylosanthes (stylo) species are economically important tropical and subtropical forage legumes. They are vulnerable to chilling and frost, and little is known about the genetic and molecular mechanisms of their responses or adaptations to low temperature stress. Two cold-responsive NAC genes, SgNAC1 and SgNAC2, were selected from a whole transcriptome profiling study of fine-stem stylo (S. guianensis var. intermedia) and further investigated for their roles in cold stress tolerance. Bioinformatic analysis indicated that SgNAC1 and SgNAC2 belonged to ATAF subgroup of NAC family, and shared highly conserved N-terminal A–E NAC subdomains and a EVQS[E/x]PK[W/I] motif with ATAF-like NAC transcription factors. Expression profiling, subcellular location and transactivation assay revealed that SgNAC1 and SgNAC2 encode nucleus-localized polypeptides with transactivation activities and responds promptly to cold stress. Phenotypic and physiological changes indicated that the transgenic tobacco plants overexpressing SgNAC1 and SgNAC2 were more tolerant to cold stress than the wild-type plants. Meanwhile, the expression of SgNAC1 and SgNAC2 were significantly enhanced at the early stages of cold treatment, indicating that overexpression of SgNAC1 and SgNAC2 is sufficient to confer cold tolerance to tobacco plants. These results suggest that SgNAC1 and SgNAC2 are promising candidate genes for cold tolerance improvement strategies in stylo.
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影响因子：
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作者：
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影响因子：
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