Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: Whole-transcriptome sequencing of peripheral blood mononuclear cells.
Markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome: Whole-transcriptome sequencing of peripheral blood mononuclear cells.
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DOI：
10.1177/0300060516652762
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
Ha IS
中科院分区：
文献类型：
--
作者：
Kang HG;Seo H;Lim JH;Kim JI;Han KH;Park HW;Koo JW;Kim KH;Kim JH;Cheong HI;Ha IS
确定小儿特发性肾病综合征的疾病标志物和类固醇反应性。对 NS 患者的外周血单核细胞 (PBMC) 进行全转录组测序。在活动性 NS 患者与缓解期患者、类固醇敏感型 NS (SSNS) 与类固醇抵抗性 NS (SRNS) 患者中鉴定出差异表达基因 (DEG)。 NS 患者 (n = 10) 与缓解期患者 (n = 9) 中总共鉴定出 1065 个 DEG。这些差异表达基因与细胞因子和/或免疫系统信号传导以及细胞外基质相关。 SSNS (n = 6) 和 SRNS (n = 4) 之间的比较确定了 1890 个 DEG。这些类固醇反应性标记物富含与细胞周期相关的基因、microRNA 的靶标以及与细胞因子相关的基因。确定了有意义的 DEG。对更多患者进行的其他研究将提供更全面的数据。
To identify markers of disease and steroid responsiveness in paediatric idiopathic nephrotic syndrome. Whole-transcriptome sequencing was performed of peripheral blood mononuclear cells (PBMCs) from patients with NS. Differentially expressed genes (DEGs) were identified in patients with active NS vs those in remission, and those with steroid-sensitive NS (SSNS) vs steroid-resistant NS (SRNS). A total of 1065 DEGs were identified in patients with NS (n = 10) vs those in remission (n = 9). These DEGs correlated with cytokine and/or immune system signalling and the extracellular matrix. Comparisons between SSNS (n = 6) and SRNS (n = 4) identified 1890 DEGs. These markers of steroid responsiveness were enriched with genes related to the cell cycle, targets of microRNAs, and genes related to cytokines. Meaningful DEGs were identified. Additional studies with larger numbers of patients will provide more comprehensive data.
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David Gene功能分类工具：一种以生物模块为中心的新型算法，可在功能上分析大基因列表。
影响因子：
12.3
作者：
Huang, Da Wei;Sherman, Brad T;Tan, Qina;Collins, Jack R;Alvord, W Gregory;Roayaei, Jean;Stephens, Robert;Baseler, Michael W;Lane, H Clifford;Lempicki, Richard A
通讯作者：
Lempicki, Richard A
影响因子：
14.9
作者：
Huang DW;Sherman BT;Tan Q;Kir J;Liu D;Bryant D;Guo Y;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA
影响因子：
13.6
作者：
Sahali, D;Pawlak, A;Guellaën, G
通讯作者：
Guellaën, G
影响因子：
14.9
作者：
Meyer LR;Zweig AS;Hinrichs AS;Karolchik D;Kuhn RM;Wong M;Sloan CA;Rosenbloom KR;Roe G;Rhead B;Raney BJ;Pohl A;Malladi VS;Li CH;Lee BT;Learned K;Kirkup V;Hsu F;Heitner S;Harte RA;Haeussler M;Guruvadoo L;Goldman M;Giardine BM;Fujita PA;Dreszer TR;Diekhans M;Cline MS;Clawson H;Barber GP;Haussler D;Kent WJ
通讯作者：
Kent WJ
DOI：
10.1038/nrg2934
发表时间：
2011-02
期刊：
Nature reviews. Genetics
影响因子：
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作者：
通讯作者：
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