Effect of dietary iron loading on recognition memory in growing rats.
Effect of dietary iron loading on recognition memory in growing rats.
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DOI：
10.1371/journal.pone.0120609
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kim J
中科院分区：
文献类型：
--
作者：
Han M;Kim J
虽然营养和神经行为问题与老年人生长过程中的铁缺乏和超负荷有关，但生长年龄铁负荷对神经行为表现的影响尚未得到充分探讨。为研究铁负荷对幼鼠记忆功能的影响，采用铁负荷饲料（10，000 mg/kg饲料）或铁充足对照饲料（50 mg/kg饲料）喂养幼鼠1个月，并进行一系列行为学测试。铁负荷的大鼠显示血清和肝脏中的非血红素铁水平升高，表明全身性铁超载的条件。在大脑中，非血红素铁升高，在前额叶皮质的铁负荷大鼠与对照组相比，而有两个饮食组之间的其他脑区的铁含量没有差异。虽然铁负荷并没有改变运动协调或焦虑样行为，铁负荷大鼠表现出更好的识别记忆，所代表的增加新的物体识别指数（从参考值增加22%）比对照组大鼠（增加12%; P=0.047）。Western blot分析显示，铁负荷大鼠前额叶皮质多巴胺受体1上调（增加142%; P=0.002）。此外，谷氨酸受体（NMDA和AMPA）和烟碱乙酰胆碱受体（nAChR）的水平显着升高，在铁负荷大鼠的前额叶皮层（NR 1增加62%，增加70%，Glu 1A，增加115%，nAChR）。膳食铁负荷也增加了海马NMDA受体和nAChR的表达。这些结果支持了铁对学习和记忆至关重要的观点，并进一步揭示了在生命的发育和快速生长时期补充铁可以改善记忆表现。我们的研究还表明，胆碱能和多巴胺能神经传递途径都受到膳食铁的调节，并为铁负荷在改善记忆中的作用提供了分子基础。
While nutritional and neurobehavioral problems are associated with both iron deficiency during growth and overload in the elderly, the effect of iron loading in growing ages on neurobehavioral performance has not been fully explored. To characterize the role of dietary iron loading in memory function in the young, weanling rats were fed iron-loading diet (10,000 mg iron/kg diet) or iron-adequate control diet (50 mg/kg) for one month, during which a battery of behavioral tests were conducted. Iron-loaded rats displayed elevated non-heme iron levels in serum and liver, indicating a condition of systemic iron overload. In the brain, non-heme iron was elevated in the prefrontal cortex of iron-loaded rats compared with controls, whereas there was no difference in iron content in other brain regions between the two diet groups. While iron loading did not alter motor coordination or anxiety-like behavior, iron-loaded rats exhibited a better recognition memory, as represented by an increased novel object recognition index (22% increase from the reference value) than control rats (12% increase; P=0.047). Western blot analysis showed an up-regulation of dopamine receptor 1 in the prefrontal cortex from iron-loaded rats (142% increase; P=0.002). Furthermore, levels of glutamate receptors (both NMDA and AMPA) and nicotinic acetylcholine receptor (nAChR) were significantly elevated in the prefrontal cortex of iron-loaded rats (62% increase in NR1; 70% increase in Glu1A; 115% increase in nAChR). Dietary iron loading also increased the expression of NMDA receptors and nAChR in the hippocampus. These results support the idea that iron is essential for learning and memory and further reveal that iron supplementation during developmental and rapidly growing periods of life improves memory performance. Our investigation also demonstrates that both cholinergic and glutamatergic neurotransmission pathways are regulated by dietary iron and provides a molecular basis for the role of iron loading in improved memory.
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影响因子：
2.5
作者：
Bartzokis, G;Beckson, M;Marder, SR
通讯作者：
Marder, SR
影响因子：
2.7
作者：
de Lima, MNM;Laranja, DC;Schröder, N
通讯作者：
Schröder, N
DOI：
10.1007/bf02867982
发表时间：
2002-07-01
期刊：
Indian journal of clinical biochemistry : IJCB
影响因子：
--
作者：
Batra, Jyoti;Seth, P K
通讯作者：
Seth, P K
影响因子：
3.3
作者：
Choi, B. -R.;Bang, S.;Chen, Y.;Cheah, J. H.;Kim, S. F.
通讯作者：
Kim, S. F.
影响因子：
3.4
作者：
de Lima, MNM;Polydoro, M;Schröder, N
通讯作者：
Schröder, N