Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic.
Investigations into SARS-CoV-2 and other coronaviruses on mink farms in France late in the first year of the COVID-19 pandemic.
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DOI：
10.1371/journal.pone.0290444
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
2020年初COVID-19疫情爆发后不久，欧洲多个国家的多个饲养美国水貂（Neovison vison）的水貂养殖场被发现感染β冠状病毒SARS-CoV-2。形成新的水库和水貂引起反向人畜共患病的风险很快成为一个主要问题。这项研究的目的是调查四个法国水貂养殖场，以确定SARS-CoV-2是否在2020年底在那里传播。调查在屠宰期间进行，因此便于不同类型的采样（拭子和血液）。在4个水貂养殖场之一，96.6%的血清样品用纯化的N蛋白重组抗原包被的SARS-CoV-2 ELISA检测呈阳性，54/162（33%）咽气管拭子用RT-qPCR检测呈阳性。从该农场测序的12个SARS-CoV-2基因组之间的遗传变异表明，在采样时，几个谱系的共循环。所有检测到的SARS-CoV-2基因组都嵌套在20 A进化枝（Nextclade）内，以及在同一时期从人类采样的SARS-CoV-2基因组。在其他三个农场，ELISA法检测的SARS-CoV-2血清阳性率在0.3%至1.1%之间。有趣的是，在这三个农场中，来自两个农场的11个咽气管拭子和3个粪便池通过终点RT-PCR检测出α冠状病毒阳性，该病毒与2015年在丹麦农场观察到的水貂冠状病毒序列非常相似。此外，在两个甲型冠状病毒阳性农场中的一个农场中发现了水貂杯状病毒科。这些隐性病毒感染的临床影响尚不清楚。水貂养殖场中SARS-CoV-2与其他病毒的共感染可能有助于解释欧洲不同受感染养殖场的临床症状多样性。此外，一种α冠状病毒和SARS-CoV-2在水貂养殖场的共同传播可能会增加α和β冠状病毒之间病毒重组的风险，正如已经在野生动物和家养动物以及人类中提出的那样。
Soon after the beginning of the COVID-19 pandemic in early 2020, the Betacoronavirus SARS-CoV-2 infection of several mink farms breeding American minks (Neovison vison) for fur was detected in various European countries. The risk of a new reservoir being formed and of a reverse zoonosis from minks quickly became a major concern. The aim of this study was to investigate the four French mink farms to see whether SARS-CoV-2 was circulating there in late 2020. The investigations took place during the slaughtering period, thus facilitating different types of sampling (swabs and blood). On one of the four mink farms, 96.6% of serum samples were positive when tested with a SARS-CoV-2 ELISA coated with purified N protein recombinant antigen, and 54 out of 162 (33%) pharyngo-tracheal swabs were positive by RT-qPCR. The genetic variability among 12 SARS-CoV-2 genomes sequenced from this farm indicated the co-circulation of several lineages at the time of sampling. All the SARS-CoV-2 genomes detected were nested within the 20A clade (Nextclade), together with SARS-CoV-2 genomes from humans sampled during the same period. The percentage of SARS-CoV-2 seropositivity by ELISA varied between 0.3 and 1.1% on the other three farms. Interestingly, among these three farms, 11 pharyngo-tracheal swabs and 3 fecal pools from two farms were positive by end-point RT-PCR for an Alphacoronavirus very similar to a mink coronavirus sequence observed on Danish farms in 2015. In addition, a mink Caliciviridae was identified on one of the two farms positive for Alphacoronavirus. The clinical impact of these inapparent viral infections is not known. The co-infection of SARS-CoV-2 with other viruses on mink farms could help explain the diversity of clinical symptoms noted on different infected farms in Europe. In addition, the co-circulation of an Alphacoronavirus and SARS-CoV-2 on a mink farm would potentially increase the risk of viral recombination between alpha and betacoronaviruses as already suggested in wild and domestic animals, as well as in humans.
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影响因子：
11.8
作者：
Rabalski L;Kosinski M;Smura T;Aaltonen K;Kant R;Sironen T;Szewczyk B;Grzybek M
通讯作者：
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影响因子：
48
作者：
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通讯作者：
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DOI：
10.1126/science.abe5901
发表时间：
2021-01-08
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
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影响因子：
4.3
作者：
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通讯作者：
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DOI：
10.3390/life11080820
发表时间：
2021-08-11
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Scarpa F;Sanna D;Azzena I;Cossu P;Giovanetti M;Benvenuto D;Coradduzza E;Alexiev I;Casu M;Fiori PL;Ciccozzi M
通讯作者：
Ciccozzi M