Immune anticipation of mating in Drosophila: Turandot M promotes immunity against sexually transmitted fungal infections.
Immune anticipation of mating in Drosophila: Turandot M promotes immunity against sexually transmitted fungal infections.
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DOI：
10.1098/rspb.2013.2018
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发表时间：
2013-12-22
期刊：
影响因子：
--
通讯作者：
Priest NK
中科院分区：
文献类型：
--
作者：
Zhong W;McClure CD;Evans CR;Mlynski DT;Immonen E;Ritchie MG;Priest NK
虽然众所周知，交配会增加感染的风险，但我们不知道女性如何减轻性传播感染（STI）的健康成本。最近的研究表明，雌性果蝇（Drosophila melanogaster）在听到雄性求偶歌时，会特异性上调免疫和应激反应基因图兰朵家族的两个成员图兰朵M和图兰朵C（TotM和TotC）。在这里，我们使用Gal 4/UAS RNAi基因敲低系统来测试这些基因的表达是否为感染昆虫病原真菌罗伯茨绿僵菌的女性在性传播下提供健身益处。作为对照，我们还检查了由Dorsal-related immunity factor（Dif）赋予的免疫力，Dif是Toll信号通路的核心成分，被认为提供针对真菌感染的免疫力。我们表明，TotM，而不是TotC或Dif，提供生存的好处，女性以下的性传播感染，但不是直接局部感染后。我们还表明，尽管TotM的表达为健康女性提供了生育力益处，但它却以生存为代价，这有助于解释为什么TotM不是组成性表达。总之，这些结果表明，TotM的预期表达促进了对真菌性传播感染的特异性免疫，并表明免疫预期比目前认识到的更常见。
Although it is well known that mating increases the risk of infection, we do not know how females mitigate the fitness costs of sexually transmitted infections (STIs). It has recently been shown that female fruitflies, Drosophila melanogaster, specifically upregulate two members of the Turandot family of immune and stress response genes, Turandot M and Turandot C (TotM and TotC), when they hear male courtship song. Here, we use the Gal4/UAS RNAi gene knockdown system to test whether the expression of these genes provides fitness benefits for females infected with the entomopathogenic fungus, Metarhizium robertsii under sexual transmission. As a control, we also examined the immunity conferred by Dorsal-related immunity factor (Dif), a central component of the Toll signalling pathway thought to provide immunity against fungal infections. We show that TotM, but not TotC or Dif, provides survival benefits to females following STIs, but not after direct topical infections. We also show that though the expression of TotM provides fecundity benefits for healthy females, it comes at a cost to their survival, which helps to explain why TotM is not constitutively expressed. Together, these results show that the anticipatory expression of TotM promotes specific immunity against fungal STIs and suggest that immune anticipation is more common than currently appreciated.
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DOI：
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发表时间：
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影响因子：
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