Nuclear receptors of the honey bee: annotation and expression in the adult brain.
Nuclear receptors of the honey bee: annotation and expression in the adult brain.
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DOI：
10.1111/j.1365-2583.2006.00679.x
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发表时间：
2006-10
影响因子：
2.6
通讯作者：
Fahrbach SE
中科院分区：
文献类型：
--
作者：
Velarde RA;Robinson GE;Fahrbach SE
果蝇基因组编码18个典型的核受体。所有果蝇的核受体都存在于蜜蜂的基因组中。考虑到果蝇和Apis谱系分化的时间是数亿年，在两个基因组中匹配的同源核受体的鉴定揭示了“制造”内生真菌昆虫所需的核受体的基本集合。唯一的新奇之处是在蜜蜂基因组中存在第三个类似于脊椎动物光受体特异性核受体（PNR）的昆虫基因。系统发育分析表明，我们将这种新基因命名为AmPNR-like，是果蝇或人类基因组中未发现的NR2亚家族的新成员。这种基因在蜜蜂发育中的复眼中表达。与脊椎动物一样，节肢动物的核受体在胚胎和胚胎后发育中起着关键作用。对果蝇的研究主要集中在这些转录因子在胚胎发生和变态中的作用。利用原位杂交和定量RT-PCR技术对成年蜜蜂大脑中表达的序列标记文库进行检测，发现核受体家族的几个成员（AmSVP、AmUSP、AmERR、AmHr46、AmFtz-F1和AmHnf-4）在成年蜜蜂大脑中表达。进一步分析蘑菇体（昆虫学习和记忆的主要大脑中心）中AmUSP和AmSVP的表达，揭示了在成虫觅食行为发展过程中转录本丰度的变化，以及在AmUSP的情况下转录本定位的变化。因此，对蜜蜂的研究为理解成人大脑中的核受体功能提供了一个模型。
The Drosophila genome encodes 18 canonical nuclear receptors. All of the Drosophila nuclear receptors are here shown to be present in the genome of the honey bee (Apis mellifera). Given that the time since divergence of the Drosophila and Apis lineages is measured in hundreds of millions of years, the identification of matched orthologous nuclear receptors in the two genomes reveals the fundamental set of nuclear receptors required to ‘make’ an endopterygote insect. The single novelty is the presence in the A. mellifera genome of a third insect gene similar to vertebrate photoreceptor-specific nuclear receptor (PNR). Phylogenetic analysis indicates that this novel gene, which we have named AmPNR-like, is a new member of the NR2 subfamily not found in the Drosophila or human genomes. This gene is expressed in the developing compound eye of the honey bee. Like their vertebrate counterparts, arthropod nuclear receptors play key roles in embryonic and postembryonic development. Studies in Drosophila have focused primarily on the role of these transcription factors in embryogenesis and metamorphosis. Examination of an expressed sequence tag library developed from the adult bee brain and analysis of transcript expression in brain using in situ hybridization and quantitative RT-PCR revealed that several members of the nuclear receptor family (AmSVP, AmUSP, AmERR, AmHr46, AmFtz-F1, and AmHnf-4) are expressed in the brain of the adult bee. Further analysis of the expression of AmUSP and AmSVP in the mushroom bodies, the major insect brain centre for learning and memory, revealed changes in transcript abundance and, in the case of AmUSP, changes in transcript localization, during the development of foraging behaviour in the adult. Study of the honey bee therefore provides a model for understanding nuclear receptor function in the adult brain.
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影响因子：
56.9
作者：
Ben-Shahar, Y;Robichon, A;Robinson, GE
通讯作者：
Robinson, GE
DOI：
10.1073/pnas.94.13.6803
发表时间：
1997-06-24
影响因子：
11.1
作者：
Escriva, H;Safi, R;Laudet, V
通讯作者：
Laudet, V
影响因子：
2.7
作者：
Gauchat, D;Escriva, H;Galliot, B
通讯作者：
Galliot, B
影响因子：
14.9
作者：
Duarte, J;Perrière, G;Robinson-Rechavi, M
通讯作者：
Robinson-Rechavi, M
DOI：
10.1016/j.cbpb.2005.04.014
发表时间：
2005-08-01
影响因子：
2.3
作者：
Elekonich, MM;Roberts, SP
通讯作者：
Roberts, SP