Mechanistic model of Rothia mucilaginosa adaptation toward persistence in the CF lung, based on a genome reconstructed from metagenomic data.
Mechanistic model of Rothia mucilaginosa adaptation toward persistence in the CF lung, based on a genome reconstructed from metagenomic data.
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基于从元基因组数据重建的基因组，Rothia粘膜粘膜对CF肺部持久性的适应性模型。
DOI：
10.1371/journal.pone.0064285
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Rohwer F
中科院分区：
文献类型：
--
作者：
Lim YW;Schmieder R;Haynes M;Furlan M;Matthews TD;Whiteson K;Poole SJ;Hayes CS;Low DA;Maughan H;Edwards R;Conrad D;Rohwer F
患有囊性纤维化（CF）的个体中受损的粘膜纤毛清除使得机会性病原体能够定殖CF肺。在这里，我们表明，粘液罗氏菌是一种常见的CF机会主义者，存在于我们的患者队列的83%，几乎与铜绿假单胞菌（89%）一样普遍。肺微生物宏基因组测序鉴定出独特的R。粘液菌株在每个病人，大概是由于肺内的演变。一个近完全R. mucilaginosa（CF 1 E）基因组阐明了CF肺的许多潜在生理适应，包括抗生素抗性、细胞外乳酸的利用和I型限制修饰系统的修饰。从宏基因组预测的代谢特征表明，R。在CF肺中，粘液菌已经适应于生活在粘液层的微需氧表面内。研究结果还突出了许多CF病原体的显着进化和生态相似性;对这些相似性的进一步研究有可能指导患者的护理和治疗。
The impaired mucociliary clearance in individuals with Cystic Fibrosis (CF) enables opportunistic pathogens to colonize CF lungs. Here we show that Rothia mucilaginosa is a common CF opportunist that was present in 83% of our patient cohort, almost as prevalent as Pseudomonas aeruginosa (89%). Sequencing of lung microbial metagenomes identified unique R. mucilaginosa strains in each patient, presumably due to evolution within the lung. The de novo assembly of a near-complete R. mucilaginosa (CF1E) genome illuminated a number of potential physiological adaptations to the CF lung, including antibiotic resistance, utilization of extracellular lactate, and modification of the type I restriction-modification system. Metabolic characteristics predicted from the metagenomes suggested R. mucilaginosa have adapted to live within the microaerophilic surface of the mucus layer in CF lungs. The results also highlight the remarkable evolutionary and ecological similarities of many CF pathogens; further examination of these similarities has the potential to guide patient care and treatment.
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