Bcr1 plays a central role in the regulation of opaque cell filamentation in Candida albicans.
Bcr1 plays a central role in the regulation of opaque cell filamentation in Candida albicans.
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Bcr1 在白色念珠菌不透明细胞丝状化的调节中发挥核心作用
DOI：
10.1111/mmi.12310
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发表时间：
2013-08
影响因子：
3.6
通讯作者：
Huang G
中科院分区：
文献类型：
--
作者：
Guan G;Xie J;Tao L;Nobile CJ;Sun Y;Cao C;Tong Y;Huang G
人类真菌病原体白色念珠菌至少有两种形态转变：白色到不透明的细胞转变和酵母到菌丝的转变。历史上，不透明细胞在标准的诱导成丝条件下没有发生成丝。本研究发现Bcr1及其下游调节因子Cup9、Nrg1和Czf1以及cAMP信号通路控制白色假丝酵母的不透明细胞丝化。我们发现，在标准培养条件下，缺失BCR1、CUP9、NRG1和CZF1会导致不透明的细胞丝化。白细胞中BCR1的破坏对菌丝生长没有明显影响，这表明BCR1在实验条件下是一种不透明的特异性丝化调节剂。此外，cAMP信号通路失活或其下游转录调控因子fl8和EFG1的破坏，显著减弱了bcr1/bcr1突变体不透明细胞中的丝化。HGC1是编码G1细胞周期蛋白的cAMP信号通路的下游基因，它的缺失完全阻断了BCR1失活诱导的不透明细胞丝化。这些结果表明，Bcr1调控的不透明细胞丝化依赖于cAMP信号通路。本研究在白色念珠菌的白色-不透明转换和酵母-丝状生长转换之间建立了联系。
The human fungal pathogen Candida albicans has at least two types of morphological transitions: white to opaque cell transitions and yeast to hyphal transitions. Opaque cells have historically not been known to undergo filamentation under standard filament‐inducing conditions. Here we find that Bcr1 and its downstream regulators Cup9, Nrg1 and Czf1 and the cAMP‐signalling pathway control opaque cell filamentation in C. albicans. We have shown that deletion of BCR1, CUP9, NRG1 and CZF1 results in opaque cell filamentation under standard culture conditions. Disruption of BCR1 in white cells has no obvious effect on hyphal growth, suggesting that Bcr1 is an opaque‐specific regulator of filamentation under the conditions tested. Moreover, inactivation of the cAMP‐signalling pathway or disruption of its downstream transcriptional regulators, FLO8 and EFG1, strikingly attenuates filamentation in opaque cells of the bcr1/bcr1 mutant. Deletion of HGC1, a downstream gene of the cAMP‐signalling pathway encoding G1 cyclin‐related protein, completely blocks opaque cell filamentation induced by inactivation of BCR1. These results demonstrate that Bcr1 regulated opaque cell filamentation is dependent on the cAMP‐signalling pathway. This study establishes a link between the white‐opaque switch and the yeast‐filamentous growth transition in C. albicans.
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影响因子：
5.2
作者：
Huang G
通讯作者：
Huang G
影响因子：
64.5
作者：
Lo, HJ;Kohler, JR;Fink, GR
通讯作者：
Fink, GR
影响因子：
4.5
作者：
Homann OR;Dea J;Noble SM;Johnson AD
通讯作者：
Johnson AD
影响因子：
9.2
作者：
Nobile, CJ;Mitchell, AP
通讯作者：
Mitchell, AP
影响因子：
5.4
作者：
Lohse, Matthew B.;Johnson, Alexander D.
通讯作者：
Johnson, Alexander D.