Optogenetic activation of CA1 pyramidal neurons at the dorsal and ventral hippocampus evokes distinct brain-wide responses revealed by mouse fMRI.
Optogenetic activation of CA1 pyramidal neurons at the dorsal and ventral hippocampus evokes distinct brain-wide responses revealed by mouse fMRI.
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DOI：
10.1371/journal.pone.0121417
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tanaka KF
中科院分区：
文献类型：
--
作者：
Takata N;Yoshida K;Komaki Y;Xu M;Sakai Y;Hikishima K;Mimura M;Okano H;Tanaka KF
背侧和腹侧海马区（dHP和vHP）被认为具有不同的功能。电生理学研究揭示了海马内的差异沿着背腹轴。然而，海马外的dHP和vHP活动的影响仍然不清楚。在这项研究中，我们比较了dHP或vHP激活时全脑反应的空间分布，并进一步估计dHP和vHP与相应的海马外区域之间的连接强度。为了实现这一目标，我们首先调查了局部场电位（LFP）和多单位活动（MUA）在光刺激麻醉转基因小鼠，其CA 1锥体神经元表达的阶跃函数视蛋白变异体通道视紫红质-2（ChR 2）海马的反应。光遗传刺激增加海马LFP功率在θ，γ，和超快频带，增强MUA，表明光诱导激活CA 1锥体神经元。使用fMRI检查的全脑反应显示，dHP或vHP的光遗传学激活引起血氧水平依赖性（BOLD）fMRI信号原位。虽然在dHP激活诱导BOLD反应在vHP，相反的情况下，没有观察到。在海马结构外，激活dHP，而不是vHP，诱发BOLD反应在压后皮质（RSP），这是符合解剖学证据。与此相反，BOLD反应在侧隔（LS）诱导只有在vHP激活，即使dHP和vHP发送轴突纤维的LS。我们的研究结果表明，dHP和vHP激活的主要目标是不同的，这与dHP和RSP在空间记忆中的归因功能以及vHP和LS在情绪反应中的归因功能一致。
The dorsal and ventral hippocampal regions (dHP and vHP) are proposed to have distinct functions. Electrophysiological studies have revealed intra-hippocampal variances along the dorsoventral axis. Nevertheless, the extra-hippocampal influences of dHP and vHP activities remain unclear. In this study, we compared the spatial distribution of brain-wide responses upon dHP or vHP activation and further estimate connection strengths between the dHP and the vHP with corresponding extra-hippocampal areas. To achieve this, we first investigated responses of local field potential (LFP) and multi unit activities (MUA) upon light stimulation in the hippocampus of an anesthetized transgenic mouse, whose CA1 pyramidal neurons expressed a step-function opsin variant of channelrhodopsin-2 (ChR2). Optogenetic stimulation increased hippocampal LFP power at theta, gamma, and ultra-fast frequency bands, and augmented MUA, indicating light-induced activation of CA1 pyramidal neurons. Brain-wide responses examined using fMRI revealed that optogenetic activation at the dHP or vHP caused blood oxygenation level-dependent (BOLD) fMRI signals in situ. Although activation at the dHP induced BOLD responses at the vHP, the opposite was not observed. Outside the hippocampal formation, activation at the dHP, but not the vHP, evoked BOLD responses at the retrosplenial cortex (RSP), which is in line with anatomical evidence. In contrast, BOLD responses at the lateral septum (LS) were induced only upon vHP activation, even though both dHP and vHP send axonal fibers to the LS. Our findings suggest that the primary targets of dHP and vHP activation are distinct, which concurs with attributed functions of the dHP and RSP in spatial memory, as well as of the vHP and LS in emotional responses.
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影响因子：
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作者：
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通讯作者：
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