Intrinsic Migratory Properties of Cultured Schwann Cells Based on Single-Cell Migration Assay
Intrinsic Migratory Properties of Cultured Schwann Cells Based on Single-Cell Migration Assay
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基于单细胞迁移测定的培养雪旺细胞的内在迁移特性
DOI：
10.1371/journal.pone.0051824
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发表时间：
2012-12
期刊：
影响因子：
3.7
通讯作者：
Zhi-hui Huang
中科院分区：
文献类型：
--
作者：
Ying Wang;Hong-Lin Teng;Zhi-hui Huang
雪旺细胞的迁移对周围神经系统的发育至关重要，对神经损伤后的再生和髓鞘再生至关重要。虽然已经确定了几个调节雪旺细胞迁移的因素，但雪旺细胞的内在迁移特性仍然难以捉摸。在这项研究中，基于单个分离雪旺细胞的延时成像，我们研究了雪旺细胞固有的迁移特性和迁移过程中体细胞易位的分子细胞骨架机制。我们发现培养的雪旺细胞表现出三种运动表型，在迁移过程中可以自发地相互转化。细胞chalasin D或Latrunculin a梯度局部破坏前导锋的F-actin聚合，诱导前导锋崩溃，进而抑制体细胞易位。此外，在迁移的雪旺细胞中，myosin II活性呈现极化分布，其前导过程的表达高于胞体和后导过程。通过正面应用ML-7或BDM（肌球蛋白II抑制剂）梯度来降低肌球蛋白II活性的前后差异，诱导了领先的前部和反向的soma易位的崩溃，而通过后部应用ML-7或BDM梯度或正面应用Caly（肌球蛋白II激活剂）梯度来增加肌球蛋白II活性的前后差异，加速了soma易位。综上所述，这些结果表明，在迁移过程中，雪旺细胞表现出可塑性的运动表型，并且依赖于f -肌动蛋白聚合的前导前延伸通过肌球蛋白II活性介导的体向前易位。
The migration of Schwann cells is critical for development of peripheral nervous system and is essential for regeneration and remyelination after nerve injury. Although several factors have been identified to regulate Schwann cell migration, intrinsic migratory properties of Schwann cells remain elusive. In this study, based on time-lapse imaging of single isolated Schwann cells, we examined the intrinsic migratory properties of Schwann cells and the molecular cytoskeletal machinery of soma translocation during migration. We found that cultured Schwann cells displayed three motile phenotypes, which could transform into each other spontaneously during their migration. Local disruption of F-actin polymerization at leading front by a Cytochalasin D or Latrunculin A gradient induced collapse of leading front, and then inhibited soma translocation. Moreover, in migrating Schwann cells, myosin II activity displayed a polarized distribution, with the leading process exhibiting higher expression than the soma and trailing process. Decreasing this front-to-rear difference of myosin II activity by frontal application of a ML-7 or BDM (myosin II inhibitors) gradient induced the collapse of leading front and reversed soma translocation, whereas, increasing this front-to-rear difference of myosin II activity by rear application of a ML-7 or BDM gradient or frontal application of a Caly (myosin II activator) gradient accelerated soma translocation. Taken together, these results suggest that during migration, Schwann cells display malleable motile phenotypes and the extension of leading front dependent on F-actin polymerization pulls soma forward translocation mediated by myosin II activity.
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DOI：
10.1083/jcb.200809008
发表时间：
2009-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pereira JA;Benninger Y;Baumann R;Gonçalves AF;Ozçelik M;Thurnherr T;Tricaud N;Meijer D;Fässler R;Suter U;Relvas JB
通讯作者：
Relvas JB
影响因子：
3.7
作者：
Mitchel JA;Hoffman-Kim D
通讯作者：
Hoffman-Kim D
肌球蛋白II在PNS和CNS髓鞘形成中具有不同的功能。
影响因子：
7.8
作者：
Wang, Haibo;Tewari, Ambika;Einheber, Steven;Salzer, James L.;Melendez-Vasquez, Carmen V.
通讯作者：
Melendez-Vasquez, Carmen V.
DOI：
10.1515/bc.2002.026
发表时间：
2002-01
期刊：
--
影响因子：
--
作者：
C. Lobsiger;V. Taylor;U. Suter
通讯作者：
C. Lobsiger;V. Taylor;U. Suter
ErbB2直接激活交换因子Dock7促进雪旺细胞迁移。
DOI：
10.1083/jcb.200709033
发表时间：
2008-04-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yamauchi J;Miyamoto Y;Chan JR;Tanoue A
通讯作者：
Tanoue A